ION-EXCHANGE CHROMATOGRAPHY

被引：70
|
作者：
Jungbauer, Alois [1 ]
Hahn, Rainer [1 ]
机构：
[1] Univ Nat Resources & Appl Life Sci, Dept Biotechnol, Vienna, Austria
来源：
GUIDE TO PROTEIN PURIFICATION, SECOND EDITION | 2009年 / 463卷
关键词：
SEPARATION; COLUMNS; RETENTION; MONOLITHS; MEDIA; MODEL;
D O I：
10.1016/S0076-6879(09)63022-6
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Ion-exchange chromatography is the most popular chromatographic method for separation of proteins. It is a versatile and generic toot and is suited for discovery of proteins, high-resolution purification, and industrial production of proteins. Separation conditions are within physiological range of salt and pH and in the most cases a native protein can be obtained. In this chapter, the guidance will be provided for binding and elution conditions and selection of stationary phases.
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页码：349 / 371
页数：23
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