Micropropagation and conservation of rare medicinal plant Wattakaka volubilis (Linn.) Stapf

被引：0

作者：

Arulanandam, L. John Peter ^{[1
]}

Kumar, S. Ghanthi ^{[1
]}

Sowmini, M. ^{[2
]}

机构：

[1] St Xavier Coll Autonomous, Ctr Biodivers & Biotechnol, Palayankottai 627002, India

[2] Noorul Islam Coll Arts & Sci, Kumaracoil 629180, Thuckalay, India

来源：

INDIAN JOURNAL OF BIOTECHNOLOGY | 2011年 / 10卷 / 02期

关键词：

Wattakaka volubilis; medicinal plant; shoot segments; mass propagation; SOMATIC EMBRYOGENESIS; DREGEA-VOLUBILIS; MULTIPLICATION; GLYCOSIDES; EXTRACT; SHOOT;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A micropropagation protocol for the important medicinal plant species Wattakaka volubilis was developed using shoot tip and nodal explants by culturing on Murashige-Skoog (MS) media fortified with 1.5 mg/L BAP ill combination with 0.7 mg/L Kn that resulted in 5.3 +/- 1.4 multiple shoots per explant. Maximum shoot length observed was 5.8 +/- 1.1 cm on media supplemented with 1.5 mg/L BAP and 0.6 mg/L Kn. The in vitro raised micro shoots produced highest percentage of rooting (85%) in MS media augmented with 1.0 mg/L IBA. The hardened plantlets were acclimatized in the green house conditions and then reintroduced in the Herbal garden attached to the Centre in which 76% of the plants survived.

引用

页码：238 / 241

页数：4

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