Comparison of a reporter assay and immunomagnetic separation real-time reverse transcription-PCR for the detection of enteroviruses in seeded environmental water samples

被引：30

作者：

Hwang, Yu-Chen

Leong, Oymon M.

Chen, Wilfred

Yates, Marylynn V. ^{[1
]}

机构：

[1] Univ Calif Riverside, Dept Environm Sci, Riverside, CA 92521 USA

[2] Univ Calif Riverside, Dept Environm Chem & Engn, Riverside, CA 92521 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 07期

关键词：

D O I：

10.1128/AEM.01758-06

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two newly developed protocols for infective virus detection were compared to the plaque assay. An immunomagnetic separation procedure coupled with real-time reverse transcription-PCR of viral nucleic acids was developed to identify intact enteroviral particles, and a reporter cell system responding to viral replication based on fluorescent resonance energy transfer for detection of infectious enteroviruses was tested. Both new procedures detected infective viruses in environmental samples at the same level as the plaque assay.

引用

页码：2338 / 2340

页数：3

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