High-throughput flow cytometry for drug discovery: principles, applications, and case studies

被引：29

作者：

Ding, Mei ^{[1
]}

Kaspersson, Karin ^{[1
]}

Murray, David ^{[2
]}

Bardelle, Catherine ^{[2
]}

机构：

[1] AstraZeneca, Innovat Med & Early Dev, Discovery Sci, Pepparedsleden 1, SE-43183 Molndal, Sweden

[2] AstraZeneca, Discovery Sci, Mereside, Alderley Pk, Macclesfield SK10 4TG, Cheshire, England

来源：

DRUG DISCOVERY TODAY | 2017年 / 22卷 / 12期

关键词：

MULTIPLEX ASSAY; IDENTIFICATION; SCREEN; INHIBITORS; ENTEROPATHY; MOLECULES; TOXICITY; PLATFORM; PROTEIN;

D O I：

10.1016/j.drudis.2017.09.005

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Flow cytometry is a technology providing multiparametric analysis of single cells or other suspension particles. High-throughput (HT) flow cytometry has become an attractive screening platform for drug discovery. In this review, we highlight the recent HT flow cytometry applications, and then focus on HT flow cytometry deployment at AstraZeneca (AZ). Practical considerations for successful HT flow cytometry assay development and screening are provided based on experience from four project case studies at AZ. We provide an overview of the scientific rationale, explain why HT flow cytometry was chosen and how HT flow cytometry assays deliver new ways to support the drug discovery process.

引用

页码：1844 / 1850

页数：7

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