The first luminal loop confers insulin responsiveness to glucose transporter 4

被引:11
|
作者
Kim, Ju Youn [1 ]
Kandror, Konstantin V. [1 ]
机构
[1] Boston Univ, Sch Med, Dept Biochem, Boston, MA 02118 USA
关键词
GLUT4; GLUCOSE-TRANSPORTER; STORAGE-VESICLES; C-TERMINUS; SORTILIN; TRAFFICKING; PROTEIN; MECHANISMS; SYNTAXIN-6;
D O I
10.1091/mbc.E11-10-0839
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Glucose transporter isoform 4 (GLUT4), is the sole glucose transporter responsible for the effect of insulin on postprandial blood glucose clearance. However, the nature of the insulin sensitivity of GLUT4 remains unknown. In this study, we replaced the first luminal loop of cellugyrin, a 4-transmembrane protein that does not respond to insulin, with that of GLUT4. The chimera protein is targeted to the intracellular insulin-responsive vesicles and is translocated to the plasma membrane upon insulin stimulation. The faithful targeting of the chimera depends on the expression of the sorting receptor sortilin, which interacts with the unique amino acid residues in the first luminal loop of GLUT4. Thus the first luminal loop may confer insulin responsiveness to the GLUT4 molecule.
引用
收藏
页码:910 / 917
页数:8
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