Liquid chromatography of josamycin propionate on poly(styrene-divinylbenzene)

An isocratic liquid chromatographic method is described using as stationary phase the very stable poly(styrene-divinylbenzene) PLRP-S (8 mu m, 1000 Angstrom). The mobile phase was acetonitrile-0.2 M phosphate buffer, pH 10.0-water (52:20:up to 100, v/v/v), delivered at a flow-rate of 1.0 ml/min. UV detection was performed at 232 nm. The column was heated at 60 degrees C. Josamycin propionate was separated from all impurities which were present in commercial samples. The main impurities were josamycin, leucomycin A4 propionate, josamycin 2',9-dipropionate, josamycin 3 ",9-dipropionate and platenomycin Al propionate. Full factorial design was applied to evaluate the robustness of the method. (C) 1998 Elsevier Science B.V. All rights reserved.