Liquid chromatography of spiramycin on poly(styrene-divinylbenzene)

被引：24
|
作者：
Liu, L [1 ]
Roets, E [1 ]
Hoogmartens, J [1 ]
机构：
[1] KATHOLIEKE UNIV LEUVEN,FAC FARMACEUT WETENSCHAPPEN,LAB FARMACEUT CHEM & ANAL GENEESMIDDELEN,B-3000 LOUVAIN,BELGIUM
来源：
JOURNAL OF CHROMATOGRAPHY A | 1997年 / 764卷 / 01期
关键词：
mobile phase composition; pharmaceutical analysis; stationary phases; LC; spiramycin; antibiotics;
D O I：
10.1016/S0021-9673(96)00876-X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An isocratic liquid chromatographic method is described using as stationary phase the very stable poly(styrene-divinylbenzene) PLRP-S (8 mu m 1000 Angstrom). The mobile phase is acetonitrile-0.2 M potassium phosphate buffer pH 9.0-water (37:5:up to 100, v/v/v), delivered at a flow-rate of 1.0 ml/min. UV detection is performed at 232 nm. Full factorial design was applied to evaluate the robustness of the method. Quantitative results of a number of commercial samples are presented.
引用
收藏
页码：43 / 53
页数：11
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