Simultaneous Determination of Aldoses and Uronic Acids of Citrus Pectin by LC with Precolumn Derivatization and UV Detection

被引：9
|
作者：
Li, Jianjun [1 ,2 ]
Sun, Jing [1 ]
Wang, Zhongfu [1 ]
Huang, Linjuan [1 ]
机构：
[1] NW Univ Xian, Life Sci Coll, Minist Educ, Key Lab Resource Biol & Biotechnol Western China, Xian 710069, Peoples R China
[2] Xi An Jiao Tong Univ, Coll Sci, Dept Chem, Xian 710049, Peoples R China
来源：
CHROMATOGRAPHIA | 2010年 / 72卷 / 9-10期
关键词：
Column liquid chromatography; Precolumn derivatization-UV detection; Aldoses and uronic acids; 3-Amino-9-ethylcarbazole; PERFORMANCE LIQUID-CHROMATOGRAPHY; MONOSACCHARIDES; GLYCOPROTEINS; SEPARATION; HPLC;
D O I：
10.1365/s10337-010-1755-y
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The simultaneous determination of aldoses and uronic acids is now available by liquid chromatography. The procedure involves acid hydrolysis followed by derivatization with 3-amino-9-ethylcarbazole, which was first employed for aldoses and uronic acids derivatization. The usefulness of this method is seen in the ability to analyze commercially available citrus pectin. The results show that citrus pectin consists of xylose, glucose, arabinose, rhamnose, galactose and galacturonic acid in molar ratios of 0.3:1.0:1.8:2.8:7.4:50.9, which was consistent with the result obtained by GC. The described method is suitable for routine analysis of pectin or other polysaccharides containing uronic acids.
引用
收藏
页码：849 / 855
页数：7
相关论文
共 50 条
  • [1] Simultaneous Determination of Aldoses and Uronic Acids of Citrus Pectin by LC with Precolumn Derivatization and UV Detection
    Jianjun Li
    Jing Sun
    Zhongfu Wang
    Linjuan Huang
    Chromatographia, 2010, 72 : 849 - 855
  • [2] Determination of Aldoses, Deoxy-aldoses and Uronic Acids Content in a Pectin-Rich Extract by RP-HPLC-FLD after p-AMBA Derivatization
    Silva, Ana S.
    Valente, I. M.
    Nunes, C.
    Coimbra, M. A.
    Guido, Luis F.
    CHROMATOGRAPHIA, 2013, 76 (17-18) : 1117 - 1124
  • [3] Determination of Aldoses, Deoxy-aldoses and Uronic Acids Content in a Pectin-Rich Extract by RP-HPLC-FLD after p-AMBA Derivatization
    Ana S. Silva
    I. M. Valente
    C. Nunes
    M. A. Coimbra
    Luís F. Guido
    Chromatographia, 2013, 76 : 1117 - 1124
  • [4] Simultaneous Determination of Various Isothiocyanates by RP-LC Following Precolumn Derivatization with Mercaptoethanol
    Wilson, Eli Adjele
    Ennahar, Said
    Zhao, Minjie
    Bergaentzle, Martine
    Marchioni, Eric
    Bindler, Francoise
    CHROMATOGRAPHIA, 2011, 73 : S137 - S142
  • [5] Simultaneous Determination of Various Isothiocyanates by RP-LC Following Precolumn Derivatization with Mercaptoethanol
    Eli Adjélé Wilson
    Saïd Ennahar
    Minjie Zhao
    Martine Bergaentzle
    Eric Marchioni
    Françoise Bindler
    Chromatographia, 2011, 73 : 137 - 142
  • [6] PRECOLUMN DERIVATIZATION OF SHORT-CHAIN CARBOXYLIC-ACIDS - UV AND ELECTROCHEMICAL DETECTION
    ROORDA, I
    GONNET, C
    ROCCA, JL
    ANALUSIS, 1982, 10 (09) : 409 - 412
  • [7] DETERMINATION OF FREE AMINO-ACIDS IN TOBACCO BY HPLC WITH FLUORESCENCE DETECTION AND PRECOLUMN DERIVATIZATION
    YANG, SS
    SMETENA, I
    CHROMATOGRAPHIA, 1993, 37 (11-12) : 593 - 598
  • [8] Simultaneous determination of amino acids in wheat by reversed phase high performance liguid chromatography with precolumn derivatization
    Chen, HP
    Chen, L
    Wei, YM
    Zheng, YL
    CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2005, 33 (12) : 1689 - 1692
  • [9] Simultaneous determination of betaine and choline using derivatization by HPLC with UV detection
    Rhee, Insook
    Paeng, Ki-Jung
    ANALYTICAL SCIENCE AND TECHNOLOGY, 2015, 28 (02): : 112 - 116
  • [10] Analysis of carbohydrates in beverages by capillary electrophoresis with precolumn derivatization and UV detection
    Cortacero-Ramírez, S
    Segura-Carretero, A
    Cruces-Blanco, C
    de Castro, MHB
    Fernández-Gutiérrez, A
    FOOD CHEMISTRY, 2004, 87 (03) : 471 - 476
12345