Development and Validation of an HPLC-MS/MS Analytical Method for Quantitative Determination of Acetylsalicylic Acid and its Metabolite Salicylic Acid in Human Blood Plasma

被引：1

作者：

Tserkovnaya, K. M. ^{[1
]}

Flisyuk, E., V ^{[1
]}

Shabrov, V. N. ^{[2
]}

机构：

[1] St Petersburg State Chem & Pharmaceut Univ, 14A Prof Popova St, Russia 197376, Russia

[2] LLC Quinta Analyt Yaroslavl, 52G Leningradskii Prosp, Yaroslavl 150045, Russia

来源：

PHARMACEUTICAL CHEMISTRY JOURNAL | 2022年 / 55卷 / 12期

关键词：

acetylsalicylic acid; human blood plasma; HPLC; MS; validation; bioequivalence;

D O I：

10.1007/s11094-022-02588-0

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

An HPLC-MS/MS analytical method for quantitative determination of acetylsalicylic acid (ASA) as an antithrombotic agent and its metabolite salicylic acid (SA) in human blood plasma was developed and validated. The optimum concentration ranges were 0.020 - 1.200 mu g/mL for ASA and 0.200 - 12.00 mu g/mL for SA. The optimum conditions for sample preparation using liquid-liquid extraction by EtOAc followed by removal of the solvent in a stream of N-2, redissolution of the dry residue (to concentrate the sample and increase the method sensitivity), chromatography, and mass-spectrometric detection were selected. The developed method could be used further for pharmacokinetic and bioequivalence studies.

引用

页码：1401 / 1406

页数：6

共 50 条

[31] An HPLC-MS/MS assay method for the determination of budesonide in human plasma
Backes, S
Hurshman, B
Maddox, H
Harris, JC
McCush, F
Heitkamp, J
Raub, M
Velagaleti, PR
Beussink, DR
DRUG METABOLISM REVIEWS, 2002, 34 : 157 - 157
[32] A sensitive HPLC-MS/MS method for the determination of dolutegravir in human plasma
Bennetto-Hood, Chantelle
Tabolt, Glenn
Savina, Paul
Acosta, Edward P.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2014, 945 : 225 - 232
[33] Validated HPLC-MS/MS method for determination of quetiapine in human plasma
Barrett, B.
Holcapek, M.
Huclova, J.
Borek-Dohalsky, V.
Fejt, P.
Memec, B.
Jelinek, I.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2007, 44 (02) : 498 - 505
[34] Direct Determination of Kynurenic Acid with HPLC-MS/MS Method in Honey
Pavlin, Anze
Pompe, Matevz
Kocar, Drago
ACTA CHIMICA SLOVENICA, 2023, 70 (02) : 274 - 280
[35] Development & Validation of HPLC/MS/MS method to quantitative determination of Sanguinarine and its major metabolite dihydrosanguinarine in human plasma and its application to measure plasma levels in patients treated with Samital
Pawar, Dilip
Banavalikar, M.
Riva, A.
Patrangolini, G.
Bombardelli, E.
Morazzoni, P.
JOURNAL OF CLINICAL PHARMACOLOGY, 2011, 51 (09): : 1339 - 1339
[36] Determination of Oseltamivir in Human Plasma by HPLC-MS/MS
A. P. Lakeev
N. Yu. Abdrashitova
O. S. Bryushinina
G. A. Frelikh
D. V. Tsuran
Yu. G. Zyuz’kova
V. V. Udut
Pharmaceutical Chemistry Journal, 2023, 57 : 116 - 122
[37] Determination of Oseltamivir in Human Plasma by HPLC-MS/MS
Lakeev, A. P.
Abdrashitova, N. Yu.
Bryushinina, O. S.
Frelikh, G. A.
Tsuran, D. V.
Zyuz'kova, Yu. G.
Udut, V. V.
PHARMACEUTICAL CHEMISTRY JOURNAL, 2023, 57 (01) : 116 - 122
[38] Quantitative determination of amisulpride in rat plasma by HPLC-MS/MS
Noh, Keumhan
Jang, Yoo-Jeong
Kwon, Kwang-il
Kim, Eunyoung
Jeong, Tae Cheon
Yun, Hwi-yeol
Kang, Wonku
ARCHIVES OF PHARMACAL RESEARCH, 2015, 38 (01) : 63 - 67
[39] Development, validation and application of an HPLC-MS/MS method for the determination of fentanyl and nor-fentanyl in human plasma and saliva
Bista, Sudeep R.
Lobb, Michael
Haywood, Alison
Hardy, Janet
Tapuni, Angela
Norris, Ross
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2014, 960 : 27 - 33
[40] HPLC-MS/MS method for troventol determination in human plasma and its application to biological samples
Nikitina, Aleksandra
Grigoriev, Alexander
Sidorova, Alla
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2017, 143 : 237 - 240

← 1 2 3 4 5 →