Comparison of real-time PCR and conventional hemi-nested PCR for the detection of Bordetella pertussis in nasopharyngeal samples

被引：18

作者：

Anderson, TP ^{[1
]}

Beynon, KA ^{[1
]}

Murdoch, DR ^{[1
]}

机构：

[1] Canterbury Hlth Labs, Microbiol Unit, Christchurch, New Zealand

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2003年 / 9卷 / 07期

关键词：

Bordetella pertussis; real-time PCR; hemi-nested PCR;

D O I：

10.1046/j.1469-0691.2003.00601.x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

We directly compared a conventional hemi-nested PCR assay with a real-time (LightCycler) PCR assay for the detection of Bordetella pertussis in nasopharyngeal samples. Of the 152 samples tested, 49 (32%) were positive by first-round conventional PCR, 56 (37%) were positive by the hemi-nested PCR assay, and 39 (26%) were positive by the real-time assay. All samples testing positive with the real-time assay were also positive by the hemi-nested assay (both first- and second-round PCR), and all culture-positive samples tested positive by both PCR assays. These results suggest that the hemi-nested assay is more sensitive than the real-time assay for detecting B. pertussis .

引用

页码：746 / 749

页数：4

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