Cloning and functional expression of the rat α2 subunit of soluble guanylyl cyclase

被引：21
|
作者：
Koglin, M [1 ]
Behrends, S [1 ]
机构：
[1] Univ Hamburg, Inst Expt & Klin Pharmakol, D-20251 Hamburg, Germany
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2000年 / 1494卷 / 03期
关键词：
soluble guanylyl cyclase; nitric oxide; enzyme isoform;
D O I：
10.1016/S0167-4781(00)00211-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Nitric oxide-sensitive guanylyl cyclase is a heterodimeric enzyme consisting of one alpha and one beta subunit. Here, we clone the first alpha (2) subunit ortholog and functionally express the cDNA in Sf-9 cells. Our data indicate a high degree of conservation of the primary sequence and functional activity of the rat at subunit. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：286 / 289
页数：4
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