A multiplex PCR assay for the detection of five human pathogenic Vibrio species and Plesiomonas

被引：15
|
作者：
Guan, Hongxia [1 ]
Xue, Panpan [2 ]
Zhou, Haijian [2 ]
Sha, Dan [1 ]
Wang, Duochun [2 ]
Gao, He [2 ]
Li, Jie [2 ]
Diao, Baowei [2 ]
Zhao, Hongqun [2 ]
Kan, Biao [2 ,3 ]
Zhang, Jingyun [2 ]
机构：
[1] Wuxi Ctr Dis Control & Prevent, Wuxi 214023, Jiangsu, Peoples R China
[2] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, 155 Changbai Rd, Beijing 102206, Peoples R China
[3] Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou 310003, Peoples R China
来源：
MOLECULAR AND CELLULAR PROBES | 2021年 / 55卷
关键词：
Multiplex PCR; Plesiomonas shigelloides; Vibrio;
D O I：
10.1016/j.mcp.2020.101689
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A multiplex PCR (mPCR) assay was established to detect five pathogenic Vibrio species and Plesiomonas shigelloides. Twelve genes were included: ompW, ctxA, rfbN, and wbfR from V. cholerae; tl, tdh, and trh from V. parahaemolyticus; toxR and vmhA from V. mimicus; toxR from V. fluvialis; vvhA from V. vulnificus; and the 23S rRNA gene from P. shigelloides. The specificity of the mPCR assay was 100% for the detection of 136 strains and the limits of detection (LoD) were 12.5-50 pg/reaction. The assay exhibited higher sensitivity than cultivation methods in the detection of APW cultures of 113 diarrhea samples. In the analysis of 369 suspected Vibrio populations from estuarine water samples, the specificity of the mPCR for V. cholerae and V. parahaemolyticus was 100% for both, while the sensitivities were 100% and 96.1%, respectively. The assay can be applied to screen enrichment cultures and suspected colonies from environmental and clinical samples.
引用
收藏
页数：6
相关论文
共 50 条
  • [21] Simultaneous detection of three wheat pathogenic fungal species by multiplex PCR
    Si Chen
    Yuan-yin Cao
    Tian-ya Li
    Xian-xin Wu
    Phytoparasitica, 2015, 43 : 449 - 460
  • [22] Simultaneous detection of three wheat pathogenic fungal species by multiplex PCR
    Chen, Si
    Cao, Yuan-yin
    Li, Tian-ya
    Wu, Xian-xin
    PHYTOPARASITICA, 2015, 43 (04) : 449 - 460
  • [23] Development of Multiplex PCR for Simultaneous Detection of Three Pathogenic Shigella Species
    Ranjbar, Reza
    Afshar, Davoud
    Mehrabi Tavana, Ali
    Najafi, Ali
    Pourali, Fatemeh
    Safiri, Zahra
    Sorouri Zanjani, Rahim
    Jonaidi Jafari, Nematollah
    IRANIAN JOURNAL OF PUBLIC HEALTH, 2014, 43 (12) : 1657 - 1663
  • [24] Multiplex PCR-DNA probe assay for the detection of pathogenic Escherichia coli
    Watterworth, L
    Topp, E
    Schraft, H
    Leung, KT
    JOURNAL OF MICROBIOLOGICAL METHODS, 2005, 60 (01) : 93 - 105
  • [25] Development of a Multiplex PCR Assay for Detection and Discrimination of Pathogenic and Saprophytic Leptospira in Water
    Vishwakarma, Archana
    Rethinavelu, Gayathri
    Pasupathi, Rathinsabapthi
    Ramya, Mohandass
    JOURNAL OF PURE AND APPLIED MICROBIOLOGY, 2021, 15 (01): : 240 - 245
  • [26] A multiplex PCR assay for simultaneous detection of four major pathogenic bacteria of ayu
    Suzuki, Kyuma
    Izumi, Shotaro
    Kumakawa, Shinji
    Sakai, Takamitu
    Nakayasu, Chihaya
    NIPPON SUISAN GAKKAISHI, 2019, 85 (03) : 340 - 342
  • [27] A multiplex PCR assay for the identification of five species of the Anopheles barbirostris complex in Thailand
    Brosseau, Laura
    Udom, Chanya
    Sukkanon, Chutipong
    Chareonviriyaphap, Theeraphap
    Bangs, Michael J.
    Saeung, Atiporn
    Manguin, Sylvie
    PARASITES & VECTORS, 2019, 12 (1)
  • [28] A multiplex PCR assay for the identification of five species of the Anopheles barbirostris complex in Thailand
    Laura Brosseau
    Chanya Udom
    Chutipong Sukkanon
    Theeraphap Chareonviriyaphap
    Michael J. Bangs
    Atiporn Saeung
    Sylvie Manguin
    Parasites & Vectors, 12
  • [29] Multiplex PCR assay for identifying five species of chigger mites in the Republic of Korea
    Lee, Hyeon Seung
    Choi, Kwang Shik
    ENTOMOLOGICAL RESEARCH, 2023, 53 (09) : 351 - 355
  • [30] Multiplex PCR Assay for Detection and Simultaneous Differentiation of Genotypes of Vibrio vulnificus Biotype 1
    Warner, Elizabeth B.
    Oliver, James D.
    FOODBORNE PATHOGENS AND DISEASE, 2008, 5 (05) : 691 - 693
12345