Universal Primer-Multiplex-Polymerase Chain Reaction (UP-M-PCR) and Capillary Electrophoresis-Laser-Induced Fluorescence Analysis for the Simultaneous Detection of Six Genetically Modified Maize Lines

被引：10

作者：

Zhang, Chunjiao ^{[1
]}

Xu, Wentao ^{[1
,2
]}

Zhai, Zhifang ^{[2
]}

Luo, Yunbo ^{[1
]}

Yan, Xinghua ^{[1
]}

Zhang, Nan ^{[2
]}

Huang, Kunlun ^{[1
,2
]}

机构：

[1] China Agr Univ, Coll Food Sci & Nutr Engn, Lab Food Safety & Mol Biol, Beijing 100083, Peoples R China

[2] Minist Agr, Supervis Inspect & Testing Ctr Genetically Modifi, Beijing 100083, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2011年 / 59卷 / 10期

关键词：

Genetically modified maize; universal primer-multiplex-polymerase chain reaction (UP-M-PCR); capillary electrophoresis-laser-induced fluorescence (CE-LIF); SEPARATION;

D O I：

10.1021/jf2008088

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

To meet the labeling and traceability requirement of genetically modified (GM) maize and their products for trade and regulation, it is essential to develop a specific detection method for monitoring the presence of GM content. In this work, six GM maize lines, including GA21, Bt11, NK603, Bt176, Mir604, and Mon810, were simultaneously detected by universal primer-multiplex-polymerase chain reaction (UP-M-PCR), and the amplicons for the six event-specific genes as well as the endogenous Ivr gene were successfully separated by the method of capillary electrophoresis-laser-induced fluorescence (CE-LIF). The UP-M-PCR method overcame the disadvantages in conventional M-PCR, such as complex manipulation, lower sensitivity, amplification disparity resulting from different primers, etc., and in combination with CE-LIF, it obtained a high sensitivity of 0.1 ng for both single and mixed DNA samples. The established method can be widely used for the qualitative identification of the GM maize lines.

引用

页码：5188 / 5194

页数：7