A review of approaches to the analysis of 3-nitrotyrosine

被引：103
|
作者：
Duncan, MW [1 ]
机构：
[1] Univ Colorado, Hlth Sci Ctr, Biochem Mass Spect Facil, Denver, CO 80262 USA
来源：
AMINO ACIDS | 2003年 / 25卷 / 3-4期
关键词：
3-nitrotyrosine; trace analysis; artifactual formation; assay validation; mass spectrometry;
D O I：
10.1007/s00726-003-0022-z
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Our understanding of in vivo tyrosine nitration has been confounded by problems associated with the analytical approaches that have been employed to quantify 3-nitroyrosine (3-NT). Trace analysis is a demanding task under the best of circumstances, but 3-NT offers some special concerns. This review examines some of these concerns and discusses approaches to ensuring that carefully validated analytical data are generated.
引用
收藏
页码：351 / 361
页数：11
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