The most likely organisms to infect clean operation wounds in hospital are Staphylococcus aureus, Pseudomonas. aeruginosa, and Escherichia coli, as with accidental wounds local treatment often sufficient. Evaluating the effects of plant extracts of Myrtus communis, Eucalyptus, and Nerium oleander on the types of bacteria, in comparison with the standard antibiotics. A total of 40 specimens were collected from surgery units of teaching Hospital, and AL-Basrah General Hospital during September 2007 to April 2008. Four disinfectants: Hibitan, Septol, providine Iodine, and savlon, plant extracts of Myrtus communis, Eucalyptus and Nerium oleander, in comparison with the standard antibiotics were testing against bacterial pathogens associated with postoperative wounds. From a total of (40) post operative wounds 72.5% are positive cultured cases, while 27.5% are negative cultured cases. S. aureus and Pseudomonas spp are bacteria in percentage (75.86), while protease is the lowest isolated from (20.68%) from all positive cases. Four disinfectants (Hibitan, Septol, Iodine, and savlon), and three plant extracts of M. communis, Eucalyptus, and N. oleander, and eight standard antibiotic were used to determine antibacterial activity. Most diluted concentration of disinfectants, lowest con of extracts, and some antibiotics are not effective on the bacterial types effects plant extracts are locate within the limits of studied antibiotics that give inhibition zones between 10 to 30 mm against all bacterial pathogens. There are variety of effects of various disinfectants in comparison with plant extracts and antibiotics against bacterial pathogens associated with postoperative wounds.