Characterization of whitefish (Coregonus lavaretus) sperm motility: effects of pH, cations and ovarian fluid

被引:0
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作者
Dietrich, Grzegorz J. [1 ]
Wojtczak, Mariola [1 ]
Dobosz, Stefan [2 ]
Kuzminski, Henryk [2 ]
Kowalski, Radoslaw [3 ]
Kotlowska, Mariola [1 ]
Ciereszko, Andrzej [1 ]
机构
[1] Polish Acad Sci, Dept Semen Biol, Inst Anim Reprod & Food Res, PL-10747 Olsztyn, Poland
[2] Inland Fisheries Inst, Dept Salmonid Res, PL-83300 Zukowo, Poland
[3] Polish Acad Sci, Inst Anim Reprod & Food Res, Dept Mol Androl, PL-10747 Olsztyn, Poland
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中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
The objective of this work was to describe the pattern of whitefish sperm movement using Computer-Assisted Sperm Analysis (CASA). We tested effects of pH, sodium, potassium and calcium ion concentrations as well as ovarian fluid on CASA sperm motility characteristics and duration of sperm motility. Our data demonstrated that mechanism of sperm motility is species-specific and unique for whitefish. The whitefish spermatozoa are generally characterized by a "erratic" swimming pattern. Increased potassium, calcium and sodium ions caused decreases in the percentage of motile sperm at relatively low concentrations. The optimum pH for whitefish sperm motility was established at pH 9.0 but spermatozoa were motile at low pH as well. Supplementation of activation solution with ovarian fluid increase the duration of movement.
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页码:159 / +
页数:3
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