High volume confinement in two-photon fluorescence correlation spectroscopy with radially polarized light

被引：1
|
作者：
Ivanov, D. [1 ]
Shcheslavskiy, V. [1 ]
Maerki, I. [1 ]
Leutenegger, M. [2 ]
Lasser, T. [1 ]
机构：
[1] Ecole Polytech Fed Lausanne, Lab Opt Biomed, CH-1015 Lausanne, Switzerland
[2] Max Planck Inst Biophys Chem, D-37077 Gottingen, Germany
来源：
SINGLE MOLECULE SPECTROSCOPY AND IMAGING II | 2009年 / 7185卷
关键词：
Fluorescence correlation spectroscopy; two-photon microscopy; total internal reflection; ultrafast laser; radial polarisation; FOCUS;
D O I：
10.1117/12.807596
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We present the results on two-photon total-internal-reflection fluorescence correlation spectroscopy. The combination of liquid crystal spatial light modulator, providing radial polarization, with ultrafast laser (picosecond Nd:GdVO4 laser) allowed us to take the advantage of nonlinear optical contrast mechanisms to suppress the side-lobe energy specific for radial polarization and reduce the effective excited volume twice compared to one-photon evanescent wave excitation in fluorescence correlation spectroscopy.
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页数：6
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