Influence of humidity on the infection of western flower thrips, Frankliniella occidentalis (Thysanoptera: Thripidae), by Beauveria bassiana

被引:13
|
作者
Mukawa, Shigeyuki [1 ]
Tooyama, Hirokazu [1 ]
Ikegami, Takafumi [1 ]
机构
[1] Ibaraki Agr Ctr, Biol Control Lab, Inst Plant Biotechnol, Kasama, Ibaraki 3190292, Japan
关键词
Beauveria bassiana; Frankliniella occidentalis; Pathogenicity; Relative humidity; Multiplication; Real-time quantitative PCR; ENTOMOPATHOGENIC FUNGUS; MICROBIAL CONTROL; TEMPERATURE; SUSCEPTIBILITY; CONIDIA; PATHOGEN;
D O I
10.1007/s13355-011-0033-2
中图分类号
Q96 [昆虫学];
学科分类号
摘要
The insecticidal activity of Beauveria bassiana GHA derived from a commercial mycoinsecticide BotaniGard ES against Frankliniella occidentalis was determined in a bioassay by dipping the female adults into a conidial suspension. The 90% lethal concentration of B. bassiana GHA was estimated to be 9.7 x 10(6) conidia/ml. The lethal times for achieving 90% mortality of thrips inoculated with a 1/500-diluted solution of BotaniGard ES and a 10(7.5) (3.16 x 10(7)) conidia/ml suspension of B. bassiana GHA were estimated to be five and six days, respectively. When the treated thrips were exposed to a high relative humidity (RH) of over 99% for various periods and then transferred to 60% RH, the requisite lengths of the high-humidity period to achieve 90% mortality of the thrips at six days after inoculation were estimated to be 46 and 47 h in BotaniGard ES and B. bassiana GHA, respectively. Fungal multiplication in the thrips was detected between 48 to 60 h after inoculation by measuring Beauveria-specific DNA in the host following inoculation with a B. bassiana GHA suspension of 10(7.5) conidia/ml using a real-time quantitative PCR. The mycelial growth in the host hemocoel was not influenced by the low-humidity condition.
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页码:255 / 264
页数:10
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