Molecular typing of canine parvovirus with PCR assay based on genetic markers of the antigenic variants

被引：0

作者：

Singh, Deepti ^{[1
]}

Verma, Amit Kumar ^{[1
]}

Kumar, Amit ^{[2
]}

Srivastava, Mukesh Kumar ^{[3
]}

机构：

[1] Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu C, Dept Vet Epidemiol & Prevent Med, Mathura 281001, Uttar Pradesh, India

[2] Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu C, Dept Vet Microbiol & Immunol, Mathura 281001, Uttar Pradesh, India

[3] Uttar Pradesh Pandit Deen Dayal Upadhayay Pashu C, Dept Vet Clin Med, Coll Biotechnol, Mathura 281001, Uttar Pradesh, India

来源：

INDIAN JOURNAL OF ANIMAL SCIENCES | 2015年 / 85卷 / 01期

关键词：

Canine parvovirus; Dog; India; Polymerase chain reaction; POLYMERASE-CHAIN-REACTION; VP2; GENE; PHYLOGENETIC ANALYSIS; STRAINS; EVOLUTION; DOGS; SEQUENCES; ISOLATE; BRAZIL; INDIA;

D O I：

暂无

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The canine parvovirus (CPV) is prevalent in India but only scanty literature is available on molecular differentiation of its strain using polymerase chain reaction. In the present study, 100 faecal samples from dogs suspected for canine parvo virus infection (gastroenteritis) were collected and processed for isolation of viral genomic DNA. Molecular test like PCR assay using 3 specific pairs of primers (pCPV-RT, pCPV-2ab and pCPV-2b) was conducted for differential diagnosis of the virus type. Out of 100 faecal samples, 63 were positive for presence of CPV, of which 54 were CPV-2b and 9 were CPV-2a, indicating the predominance of CPV-2b virus type.

引用

页码：12 / 15

页数：4

共 50 条

[31] A multiplex TaqMan real-time PCR for detection and differentiation of four antigenic types of canine parvovirus in China
Sun, Yaru
Cheng, Yuening
Lin, Peng
Yi, Li
Tong, Mingwei
Cao, Zhigang
Wang, Gaili
Li, Shuang
Cheng, Shipeng
Yuan, Wanzhe
Wang, Jianke
MOLECULAR AND CELLULAR PROBES, 2018, 38 : 7 - 12
[32] Molecular typing of Pyrus based on RAPD markers
Oliveira, CM
Mota, M
Monte-Corvo, L
Goulao, L
Silva, DM
SCIENTIA HORTICULTURAE, 1999, 79 (3-4) : 163 - 174
[33] Peptide nucleic acid-based (PNA) array for the antigenic discrimination of canine parvovirus
An, Dong-Jun
Jeong, Wooseog
Jeoung, Hye-Young
Lee, Myoung-Heon
Park, Jee-Yong
Lim, Ji-Ae
Park, Bong-Kyun
RESEARCH IN VETERINARY SCIENCE, 2012, 93 (01) : 515 - 519
[34] Polymorphism of molecular genetic markers (electrophoretic variants of proteins, ISSR-PCR) in representatives of Ungulata and Delphinidae
T. V. Nagornyuk
V. I. Glazko
Russian Agricultural Sciences, 2007, 33 (4) : 264 - 267
[35] GENETIC DIVERSITY ANALYSIS OF TAGETES SPECIES USING PCR BASED MOLECULAR MARKERS
Shahzadi, Irum
Ahmad, Raza
Waheed, Ummara
Shah, Mohammad Maroof
PAKISTAN JOURNAL OF BOTANY, 2016, 48 (04) : 1593 - 1599
[36] Multiplex PCR assay for the detection of genetic variants of α1-antitrypsin
Rieger, S
Riemer, H
Mannhalter, C
CLINICAL CHEMISTRY, 1999, 45 (05) : 688 - 690
[37] A rapid and highly reliable field-based LAMP assay of canine parvovirus
Parthiban, M.
Divya, Kurunchi C.
Kumanan, K.
Bargavi, D. S.
ACTA VIROLOGICA, 2012, 56 (01) : 71 - 74
[38] Evaluation of a gp63-PCR Based Assay as a Molecular Diagnosis Tool in Canine Leishmaniasis in Tunisia
Guerbouj, Souheila
Djilani, Fattouma
Bettaieb, Jihene
Lambson, Bronwen
Diouani, Mohamed Fethi
Ben Salah, Afif
Ben Ismail, Riadh
Guizani, Ikram
PLOS ONE, 2014, 9 (08):
[39] Molecular characterisation and genetic diversity of canine parvovirus type 2 prevalent in Central China
Hu, Wen
Xu, Xin
Liu, Qiang
Ji, Jun
Kan, Yunchao
Yao, Lunguang
Bi, Yingzuo
Xie, Qingmei
JOURNAL OF VETERINARY RESEARCH, 2020, 64 (03) : 347 - 354
[40] A real-time PCR assay for rapid detection and quantitation of canine parvovirus type 2 in the feces of dogs
Decaro, N
Elia, G
Martella, V
Desario, C
Campolo, M
Di Trani, L
Tarsitano, E
Tempesta, M
Buonavoglia, C
VETERINARY MICROBIOLOGY, 2005, 105 (01) : 19 - 28

← 1 2 3 4 5 →