Generation of Brachyury-mCherry knock-in reporter human pluripotent stem cell line (SNUe003-A-2) using CRISPR/CAS9 nuclease

被引：2

作者：

Jung, Juwon ^{[1
]}

Hwang, Sunsook ^{[2
]}

Seol, Hyewon ^{[1
]}

Kim, A-Hyeon ^{[3
]}

Lee, Ha Myoung ^{[3
]}

Sung, Jin Jea ^{[4
]}

Jeong, Seung Min ^{[2
]}

Choi, Young Min ^{[1
]}

Jun, Jong Kwan ^{[1
]}

Kim, Dong-wook ^{[4
]}

Jang, Jiho ^{[3
]}

机构：

[1] Seoul Natl Univ, Dept Obstet & Gynecol, Inst Reprod Med & Populat, Med Res Ctr,Coll Med, Seoul 03080, South Korea

[2] Catholic Univ Korea, Coll Med, Inst Aging & Metab Dis, Dept Biochem, 222 Banpo Daero, Seoul 06591, South Korea

[3] Catholic Kwandong Univ, Coll Med Convergence, Dept Biomed Sci, Gangneung Si, Gangwon Do, South Korea

[4] Yonsei Univ, Coll Med, Dept Physiol, 50-1 Yonsei Ro, Seoul 03722, South Korea

来源：

STEM CELL RESEARCH | 2021年 / 53卷

基金：

新加坡国家研究基金会;

关键词：

D O I：

10.1016/j.scr.2021.102321

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Brachyury is an embryonic nuclear transcription factor required for mesoderm formation and differentiation. Here, we introduced an mCherry reporter into the C terminus of Brachyury in the human pluripotent stem cell line SNUhES3 using the CRISPR/Cas9 nuclease approach. Successful gene editing was verified by DNA sequencing. SNUhES3-Brachyury-mCherry cells expressed pluripotent stem cell markers, exhibited a normal karyotype, and could generate all three germ layers. This cell line expressed the red fluorescence protein mCherry upon the induction of mesoderm differentiation. This reporter cell line could be used to monitor mesodermal population enrichment during mesodermal differentiation.

引用

页数：4

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