Generation of iPSC lines from peripheral blood mononuclear cells from 5 healthy adults

被引：31
|
作者：
Vlahos, Katerina [1 ]
Sourris, Koula [1 ]
Mayberry, Robyn [1 ]
McDonald, Penelope [1 ]
Bruveris, Freya F. [1 ,2 ]
Schiesser, Jacqueline V. [1 ,2 ]
Bozaoglu, Kiymet [1 ,2 ,3 ]
Lockhart, Paul J. [1 ,2 ,3 ]
Stanley, Edouard G. [1 ,2 ,4 ]
Elefanty, Andrew G. [1 ,2 ,4 ]
机构：
[1] Royal Childrens Hosp, Murdoch Childrens Res Inst, Melbourne, Vic 3052, Australia
[2] Univ Melbourne, Dept Paediat, Parkville, Vic 3052, Australia
[3] Murdoch Childrens Res Inst, Bruce Lefroy Ctr Genet Hlth Res, Parkville, Vic 3052, Australia
[4] Monash Univ, Dept Anat & Dev Biol, Clayton, Vic 3800, Australia
来源：
STEM CELL RESEARCH | 2019年 / 34卷
基金：
英国医学研究理事会;
关键词：
PLURIPOTENT STEM-CELLS; DIFFERENTIATION; MESODERM;
D O I：
10.1016/j.scr.2018.101380
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
We describe the generation and characterization of 5 human induced pluripotent stem cell (iPSC) lines derived from peripheral blood mononuclear cells (PBMCs) of healthy adult individuals. The PBMCs were reprogrammed using non-integrating Sendai viruses containing the reprogramming factors POU5F1 (OCT4), SOX2, KLF4 and MYC. The iPSC lines exhibited a normal karyotype, expressed pluripotency markers and differentiated into cells representative of the three embryonic germ layers. These iPSC lines can be used as controls in studying disease mechanisms.
引用
收藏
页数：5
相关论文
共 50 条
  • [1] Generation of iPSC lines from peripheral blood mononuclear cells from five healthy donors
    Vlahos, Katerina
    Sourris, Koula
    Kuah, Jia Yi
    Graham, Alison
    Suter, Annabelle
    Howden, Sara E.
    Stanley, Edouard G.
    Elefanty, Andrew G.
    STEM CELL RESEARCH, 2023, 69
  • [2] A protocol for the generation of patient-specific iPSC lines from peripheral blood mononuclear cells
    Gao, Langping
    Wang, Fangqin
    Wang, Yan
    Hu, Lidan
    Mao, Jianhua
    STAR PROTOCOLS, 2022, 3 (03):
  • [3] Generation of seven iPSC lines from peripheral blood mononuclear cells suitable to investigate Autism Spectrum Disorder
    Bozaoglu, Kiymet
    Gao, Yujing
    Stanley, Edouard
    Fanjul-Fernandez, Miriam
    Brown, Natasha J.
    Pope, Kate
    Green, Cherie C.
    Vlahos, Katerina
    Sourris, Koula
    Bahlo, Melanie
    Delatycki, Martin
    Scheffer, Ingrid
    Lockhart, Paul J.
    STEM CELL RESEARCH, 2019, 39
  • [4] Generation of four iPSC lines from peripheral blood mononuclear cells (PBMCs) of an attention deficit hyperactivity disorder (ADHD) individual and a healthy sibling in an Australia-Caucasian family
    Tong, Janette
    Lee, Kyung Min
    Liu, Xiaodong
    Nefzger, Christian M.
    Vijayakumar, Prasidhee
    Hawi, Ziarih
    Pang, Ken C.
    Parish, Clare L.
    Polo, Jose M.
    Bellgrove, Mark A.
    STEM CELL RESEARCH, 2019, 34
  • [5] Efficient generation of induced pluripotent stem cell lines from healthy donors' peripheral blood mononuclear cells of different genders
    Gao, Yunfeng
    Cheng, Yanhong
    Lin, Bao
    Xiao, Dan
    Wen, Junjie
    Xu, Jingyu
    Zheng, Ran
    Zhang, Mengna
    Cai, Cheguo
    Hu, Junyuan
    STEM CELL RESEARCH, 2024, 77
  • [6] Efficient generation of induced pluripotent stem cell lines from peripheral blood mononuclear cells
    Gao, Yunfeng
    Cheng, Yanhong
    Wen, Junjie
    Xiao, Dan
    Xu, Jingyu
    Cai, Cheguo
    Hu, Junyuan
    STEM CELL RESEARCH, 2023, 69
  • [7] Direct Generation of Immortalized Erythroid Progenitor Cell Lines from Peripheral Blood Mononuclear Cells
    Bagchi, Abhirup
    Nath, Aneesha
    Thamodaran, Vasanth
    Ijee, Smitha
    Palani, Dhavapriya
    Rajendiran, Vignesh
    Venkatesan, Vigneshwaran
    Datari, Phaneendra
    Pai, Aswin Anand
    Janet, Nancy Beryl
    Balasubramanian, Poonkuzhali
    Nakamura, Yukio
    Srivastava, Alok
    Mohankumar, Kumarasamypet Murugesan
    Thangavel, Saravanabhavan
    Velayudhan, Shaji R.
    CELLS, 2021, 10 (03) : 1 - 18
  • [8] Generation of canine dendritic cells from peripheral blood mononuclear cells
    Yoshida, H
    Momoi, Y
    Taga, N
    Ide, K
    Yamazoe, K
    Iwasaki, T
    Kudo, T
    JOURNAL OF VETERINARY MEDICAL SCIENCE, 2003, 65 (06): : 663 - 669
  • [9] Generation of neuronal cells from human peripheral blood mononuclear cells
    Horschitz, Sandra
    Meyer-Lindenberg, Andreas
    Schloss, Patrick
    NEUROREPORT, 2010, 21 (03) : 185 - 190
  • [10] Generation of a human iPSC line (CHCMUi002-A) from peripheral blood mononuclear cells from a chronic granulomatous disease patient
    Zeng, Anle
    Ni, Ke
    Fang, Shuyu
    Yu, Lang
    Gao, Yelei
    Zhou, Lina
    Zhao, Xiaodong
    STEM CELL RESEARCH, 2024, 74
12345