Efficient generation of induced pluripotent stem cell lines from healthy donors' peripheral blood mononuclear cells of different genders

被引：0
|
作者：
Gao, Yunfeng [1 ]
Cheng, Yanhong [1 ]
Lin, Bao [1 ]
Xiao, Dan [1 ]
Wen, Junjie [1 ]
Xu, Jingyu [1 ]
Zheng, Ran [1 ]
Zhang, Mengna [1 ]
Cai, Cheguo [1 ]
Hu, Junyuan [1 ]
机构：
[1] Shenzhen Beike Biotechnol Co Ltd, 59 Gaoxin South 9th Rd,Nanshan Dist Sci & Technol, Shenzhen 518000, Guangdong, Peoples R China
来源：
STEM CELL RESEARCH | 2024年 / 77卷
关键词：
Peripheral blood mononuclear cell; Reprogramming; Induced pluripotent stem cell; Triploblastic differentiation;
D O I：
10.1016/j.scr.2024.103421
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Peripheral blood mononuclear cell (PBMC) are recognized as a conveniently collected reprogramming resource. Several methods are available in academia to reprogram PBMC into induced pluripotent stem cells (iPSC). In this research, we reprogrammed PBMC of different genders by using non-integrative non-viral liposome electrotransfer containing the reprogramming factors OCT4, SOX2, KLF4, and c-MYC. The three obtained iPSC cell lines were karyotypically normal and showed significant tritiated differentiation potential in vitro and in vivo. Our study provided an efficient procedure for reprogramming PBMC into iPSC and obtained three well-functioning iPSC, that may contribute to advance personalized cell therapy in the future.
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页数：5
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