A New Real-Time Reverse Transcription-PCR Assay for Detection of Human Enterovirus 68 in Respiratory Samples

被引：21
|
作者：
Piralla, Antonio [1 ]
Girello, Alessia [1 ]
Premoli, Marta [1 ]
Baldanti, Fausto [1 ,2 ]
机构：
[1] Fdn IRCCS Policlin San Matteo, Mol Virol Unit, Microbiol & Virol Dept, Pavia, Italy
[2] Univ Pavia, Microbiol Sect, Dept Clin Surg Diagnost & Pediat Sci, I-27100 Pavia, Italy
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2015年 / 53卷 / 05期
关键词：
ILLNESS;
D O I：
10.1128/JCM.03691-14
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A global reemergence of human enterovirus 68 (EV-D68) associated with severe respiratory illness occurred in 2014. We developed and validated an EV-D68-specific real-time reverse transcription-PCR (RT-PCR) for the detection of EV-D68 in respiratory samples. The rapid diagnosis of EV-D68 may contribute to better management of EV-D68 infections.
引用
收藏
页码：1725 / 1726
页数：2
相关论文
共 50 条
  • [41] Validation of a Real-time PCR Assay for the Detection of Legionella Species in Respiratory Samples
    Cunningham, S. A.
    Sloan, L. M.
    Uhl, J. R.
    Nyre, L. M.
    Vetter, E. A.
    Cockerill, F. R.
    Patel, R.
    JOURNAL OF MOLECULAR DIAGNOSTICS, 2009, 11 (06): : 642 - 642
  • [42] Detection of SARS coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-PCR assays
    Poon, LLM
    Chan, KH
    Wong, OK
    Cheung, TKW
    Ng, I
    Zheng, BJ
    Seto, WH
    Yuen, KY
    Guan, Y
    Peiris, JSM
    CLINICAL CHEMISTRY, 2004, 50 (01) : 67 - 72
  • [43] Detection of murine norovirus 1 by using plaque assay, transfection assay, and real-time reverse transcription-PCR before and after heat exposure
    Baert, Leen
    Wobus, Christiane E.
    Van Coillie, Els
    Thackray, Larissa B.
    Debevere, Johan
    Uyttendaele, Mieke
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (02) : 543 - 546
  • [44] Performance of the RealStar Chikungunya Virus Real-Time Reverse Transcription-PCR Kit
    Panning, Marcus
    Hess, Markus
    Fischer, Waldemar
    Grywna, Klaus
    Pfeffer, Martin
    Drosten, Christian
    JOURNAL OF CLINICAL MICROBIOLOGY, 2009, 47 (09) : 3014 - 3016
  • [45] Evaluation of a Real-Time Reverse Transcription-PCR (RT-PCR) Assay for Detection of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Clinical Samples from an Outbreak in South Korea in 2015
    Lee, Jee-Soo
    Ahn, Ji Soo
    Yu, Byeong Su
    Cho, Sung Im
    Kim, Man Jin
    Choi, Jong Moon
    Seo, Soo Hyun
    Park, Sung Sup
    Seong, Moon-Woo
    JOURNAL OF CLINICAL MICROBIOLOGY, 2017, 55 (08) : 2554 - 2555
  • [46] Concentration and detection of caliciviruses in water samples by reverse transcription-PCR
    Huang, PW
    Laborde, D
    Land, VR
    Matson, DO
    Smith, AW
    Jiang, X
    APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (10) : 4383 - 4388
  • [47] Quantitative detection of Citrus tristeza virus in citrus and aphids by real-time reverse transcription-PCR (TaqMan®)
    Saponari, Maria
    Manjunath, Keremane
    Yokomi, Raymond K.
    JOURNAL OF VIROLOGICAL METHODS, 2008, 147 (01) : 43 - 53
  • [48] Detection of human herpesvirus 6 by reverse transcription-PCR
    Norton, RA
    Caserta, MT
    Hall, CB
    Schnabel, K
    Hocknell, P
    Dewhurst, S
    JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (11) : 3672 - 3675
  • [49] Real-time reverse transcription-PCR assay for future management of ERBB2-based clinical applications
    Bièche, I
    Onody, P
    Laurendeau, I
    Olivi, M
    Vidaud, D
    Lidereau, R
    Vidaud, M
    CLINICAL CHEMISTRY, 1999, 45 (08) : 1148 - 1156
  • [50] Detection and quantification of Plum pox potyvirus in aphid vectors by real-time fluorescent reverse transcription-PCR
    Schneider, WL
    Sherman, DJ
    Stone, AL
    Damsteegt, VD
    Frederick, RD
    PROCEEDINGS OF THE XIXTH INTERNATIONAL SYMPOSIUM ON VIRUS AND VIRUS-LIKE DISEASES OF TEMPERATE FRUIT CROPS: FRUIT TREE DISEASES, 2004, (657): : 135 - 139
12345