Radiation Protection of Carboxymethylation of an Acid Polysaccharides Extracted from Auricularia Auricula Against Uvb in Vitro

被引:1
|
作者
Zhang, Hua [1 ]
Wang, Zhen-Yu [1 ]
Fan, Zi-luan [1 ]
Yang, Xin [1 ]
Wang, Xue [3 ]
Zhang, Ning [2 ]
机构
[1] Harbin Inst Technol, Sch Food Sci & Engn, Harbin 150006, Peoples R China
[2] Northeast Forestry Univ, Sch Forestry, Harbin, Peoples R China
[3] Syrnrise Co Ltd, Shanghai, Peoples R China
关键词
acid Auricularia auricula polysaccharide(AAAP); carboxymethylation acid Auricularia auricula polysaccharide(C AAAP); radiation protection; UVB; in vitro; GANODERMA-LUCIDUM; MUSHROOMS;
D O I
10.4028/www.scientific.net/AMR.365.199
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
This paper reports on a water-soluble acid polysaccharide (AAP) and in how it was extracted from Auricularia auricular, acquired by CTAB, and prepared it's carboxymethylation. Chemical characterization by high-performance liquid chromatography/gel permeation chromatograph (HPLC/GPC), Fourier transform infrared (FT-IR) spectrometer and gas chromatography-mass spectrophotometer (GC MS) were investigated. Chemical analysis indicated that C AA AP was composed of arabinose, xylose, mannose, glucose and galactose, with the molar ratio at 0.04: 0.13: 1.00: 0.59: 0.29. Moreover, radiation protection against UVB in vitro indicated that at the dose range of 200-500 mu g/mL, C AAAP enhanced the protection of HepG2 cells against UVB cytotoxicity than AAAP. However, but at the dose range of 50-150 mu g/mL the result was just opposite.
引用
收藏
页码:199 / +
页数:3
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