Isolation, Purification and Bioactivity of Polysaccharides from Cirsium japonicum DC

被引：0
|
作者：
Chen, Xiao-Jun [1 ]
Liu, Liu [1 ]
Qin, Hua-Xia [1 ]
You, Xiu-Ling [1 ]
Shen, Jiang-Hong [1 ]
Liao, Zhi-Yong [1 ]
机构：
[1] Wenzhou Univ, Coll Life & Environm Sci, Zhejiang Prov Key Lab Water Environm & Marine Bio, Wenzhou 325035, Peoples R China
来源：
PROCEEDINGS OF THE 2015 INTERNATIONAL CONFERENCE ON MEDICINE AND BIOPHARMACEUTICALS | 2016年
关键词：
CANCER; HISPIDULIN;
D O I：
暂无
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
In this study, polysaccharides Cirsium japonicum DC (CJP) were extracted using both cold and hot water. CJP was then purified by ion exchange resin and chromatography. Results showed that it contained 9.64% of uronic acid and 2.64% of protein. Structural analysis indicated that CJP contained several polysaccharides with different molecular weight of 74.1 kD, 47.0 kD, 5.2 kD and 3.0 kD respectively. Furthermore, HPLC analysis showed that CJP was mainly composed of the following eight monosaccharides: Man (5.50%), Rha (6.73%), GlcA (1.14%), GalA (7.57%), Glc (21.00%), Gal (25.32%), Xyl (3.98%), and Ara (28.75%). Moreover, luciferase report gene assay demonstrated that CJP promoted the activation of both p53 and Rb, two genes playing important role in the development of tumor, indicating a potential antitumor activity for CJP.
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收藏
页码：1072 / 1081
页数：10
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