Cloning and Sequence Analysis of Rabbit Interleukin-10 (IL-10) Gene Promoter

被引：0
|
作者：
Mao Liu-Liu [1 ,3 ]
Pan Yu-Lai [2 ]
Wan Xiao-Ying [1 ,3 ]
Wang Xiao-Ming [1 ,3 ]
Feng Kai [1 ,3 ]
Zhang Lei [1 ,3 ]
He Meng-Jie [1 ,3 ]
Wu Xin-Sheng [1 ,3 ]
机构：
[1] Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Jiangsu, Peoples R China
[2] Jinling Rabbits Farm, Nanjing 211103, Peoples R China
[3] Yangzhou Univ, Jiangsu Key Lab Anim Genet & Breeding & Mol Desig, Yangzhou 225009, Jiangsu, Peoples R China
来源：
JOURNAL OF ANIMAL AND VETERINARY ADVANCES | 2012年 / 11卷 / 03期
关键词：
IL-10; promoter; transcription factor; bioinformatics; gene; China; TRANSCRIPTIONAL REGULATION; RECEPTOR;
D O I：
暂无
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
To clone the IL-10 promoter of rabbit and analyse its sequence, this study amplified the 5 flanking region of rabbit IL-10, compared the genomes and used bioinformatics software to make a forecast. Then, the researchers comfimied partial promoter sequences and analysed sonic transcription factors of the IL-10 gene promoter. The study showed that rabbit and human IL-10 gene promoter had some similarities which all cotained GAGA, CAAT and TATA. The transcriptional start site started from -80 bp of translation initiation codon (ATG) upstream region. The promoter also had some transcription factors such as AP-1, Myogenin, c-Ets-1, C/EBP alpha and so on, Results of this study predicted partial sequences, transcriptional start site and some transcriptional regulating factors binding sites of IL-10 gene promoter which would lay the foundation of lucubrating the expression and regulation of rabbit IL-10 gene.
引用
收藏
页码：323 / 326
页数：4
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