Agrobacterium-mediated transformation of cork oak (Quercus suber L.) somatic embryos

被引：9
|
作者：
Sánchez, N
Manzanera, JA
Pintos, B
Bueno, MA
机构：
[1] Inst Nacl Invest & Tecnol Agr & Alimentaria, Madrid 28040, Spain
[2] Univ Politecn Madrid, ETSI Montes, E-28040 Madrid, Spain
来源：
NEW FORESTS | 2005年 / 29卷 / 02期
关键词：
genetic transformation; hygromycin resistance; marker gene; reporter gene;
D O I：
10.1007/s11056-005-0208-1
中图分类号：
S7 [林业];
学科分类号：
0829 ; 0907 ;
摘要：
A simple method is presented for the unreported genetic transformation of cork oak (Quercus suber L.). Pro-embryo masses (PEMs) were induced on immature zygotic embryos applied to medium supplemented with 2.3 mu M 2,4-dichlorophenoxyacetic acid. The established PEMs were inoculated with Agrobacterium tumefaciens LBA4404/p35S GUS INT/pCAMBIA 1301 strain. Transformants were selected on hygromycin (IjYG) 94 mu M-supplemented medium. Viable embryos constituted 13% of those selected on HYG during 4 months. Expression of beta-glucuronidase at 4 months following co-cultivation confirmed transformation in 5.8% embryos selected on HYG. This method forms a basis for genetic transformation of cork oak somatic embryos.
引用
收藏
页码：169 / 176
页数：8
相关论文
共 50 条
  • [31] Effects of cadmium on cork oak (Quercus suber L.) plants grown in hydroponics
    Gogorcena, Yolanda
    Larbi, Ajmi
    Andaluz, Sofia
    Carpena, Ramon O.
    Abadia, Anunciacion
    Abadia, Javier
    TREE PHYSIOLOGY, 2011, 31 (12) : 1401 - 1412
  • [32] Lateral root development in a woody plant, Quercus suber L. (cork oak)
    2000, NRC Can, Ottawa, Ont, Can (78):
  • [33] Catkin frost damage in Mediterranean cork-oak (Quercus suber L.)
    García-Mozo, H
    Hidalgo, PJ
    Galán, C
    Gómez-Casero, MT
    Domínguez, E
    ISRAEL JOURNAL OF PLANT SCIENCES, 2001, 49 (01) : 41 - 47
  • [34] Lateral root development in a woody plant, Quercus suber L. (cork oak)
    Verdaguer, D
    Casero, PJ
    Molinas, M
    CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE, 2000, 78 (09): : 1125 - 1135
  • [35] CORK FROM QUERCUS SUBER L.: FOREST CERTIFICATION SYSTEM FOR SUSTAINABLE MANAGEMENT OF CORK OAK FORESTS
    Diaz-Maroto, Ignacio J.
    Diaz-Maroto, Maria Consuelo
    WOOD RESEARCH, 2020, 65 (05) : 855 - 863
  • [36] Cork from Quercus suber L.: Forest certification system for sustainable management of cork oak forests
    Diaz-Maroto I.J.
    Diaz-Maroto M.C.
    Wood Research, 2020, 65 (05): : 855 - 864
  • [37] Vegetative propagation of Quercus suber L. by somatic embryogenesisII. Plant regeneration from selected cork oak trees
    I. Hernández
    C. Celestino
    J. Alegre
    M. Toribio
    Plant Cell Reports, 2003, 21 : 765 - 770
  • [38] Developmental anatomy and apical organization of the primary root of cork oak (Quercus suber L.)
    Verdaguer, D
    Molinas, M
    INTERNATIONAL JOURNAL OF PLANT SCIENCES, 1999, 160 (03) : 471 - 481
  • [39] Radial variation of vessel size and distribution in cork oak wood (Quercus suber L.)
    Leal, Sofia
    Sousa, Vicelina B.
    Pereira, Helena
    WOOD SCIENCE AND TECHNOLOGY, 2007, 41 (04) : 339 - 350
  • [40] Sexual reproduction in the cork oak (Quercus suber L.). I. The progamic phase
    Leonor C. Boavida
    M. Carolina Varela
    J. A. Feijó
    Sexual Plant Reproduction, 1999, 11 : 347 - 353
12345