Effects of c-myb antisense RNA on TGF-β1 and α1-I collagen expression in cultured hepatic stellate cells

被引:13
|
作者
Ma, Hui-Hui [1 ]
Yao, Ji-Lu [1 ]
Li, Gang [1 ]
Yao, Chun-Lan [1 ]
Chen, Xue-Juan [1 ]
Yang, Shao-Ji [1 ]
机构
[1] Sun Yat Sen Univ, Affiliated Hosp 3, Dept Infect Dis, Guangzhou 510630, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
D O I
10.3748/wjg.v10.i24.3662
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
AIM: To investigate the effects of c-myb antisense RNA on cell proliferation and the expression of c-myb, TGF-beta 1 and alpha 1-I collagen in cultured hepatic stellate cells (HSC) from rats. METHODS: Recombinant retroviral vector of c-myb antisense gene (pDOR-myb) was constructed, and then transfected into retroviral package cell line PA317 by means of DOTAP. The pseudoviruses produced from the resistant PA317 cells were selected with G418 to infect HSCs isolated from rat livers. The cell proliferation was measured by 3-[4,5-Dimethylthiazolzyl]-2, 5-diphenyl tetrazo-dium bromide (MTT) method. The expression of c-myb, alpha(1)-I collagen and TGF-beta 1 mRNA, and c-myb protein in HSCs was detected with semi-quantitive reverse transeription-polymerase chain reaction (RT-PCR) and Western-blot respectively. RESULTS: HSCs from rats were isolated successfully with the viability >98%. In the pDOR-myb infected HSCs, the c-myb protein expression, cell proliferation, and alpha(1)-I collagen and TGF-beta 1 mRNA expression were repressed significantly compared with their corresponding control groups (P<0.01). CONCLUSION: c-myb plays a key role in activation and proliferation of HSC. c-myb antisense RNA can inhibit cell proliferation, alpha(1)-I collagen and TGF-beta 1 mRNA expression, suggesting that inhibition of c-myb gene expression might be a potential way for the treatment of liver fibrosis.
引用
收藏
页码:3662 / 3665
页数:4
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