Co-Loading of Ascorbic Acid and Tocopherol in Eudragit-Nutriosomes to Counteract Intestinal Oxidative Stress

被引：18
|
作者：
Rezvani, Maryam [1 ,2 ]
Manca, Maria Letizia [1 ]
Caddeo, Carla [1 ]
Escribano-Ferrer, Elvira [3 ]
Carbone, Claudia [4 ]
Esteban Penis, Jose [5 ]
Usach, Iris [5 ]
Diez-Sales, Octavio [5 ,6 ]
Fadda, Anna Maria [1 ]
Manconi, Maria [1 ]
机构：
[1] Univ Cagliari, Dept Sci Vita & Ambiente, Via Osped 72, I-09124 Cagliari, Italy
[2] Univ Tabriz, Dept Food Sci & Technol, Fac Agr, Tabriz 5166616471, Iran
[3] Univ Barcelona, Dept Pharm & Pharmaceut Technol & Phys Chem, Fac Pharm & Food Sci, Inst Nanosci & Nanotechnol IN2UB, Av Joan 23 S-N, E-08028 Barcelona, Spain
[4] Univ Catania, Dept Sci Farmaco, Viale A Doria 6, I-95125 Catania, Italy
[5] Univ Valencia, Dept Pharm Pharmaceut Technol & Parasitol, Avda Vicente Andres Estelles S-N, E-46100 Valencia, Spain
[6] Univ Valencia, Inst Reconocimiento Mol & Desarrollo Tecnol, Ctr Mixto Univ Politecn Valencia, Avda Vicente Andres Estelles S-N, E-46100 Valencia, Spain
来源：
PHARMACEUTICS | 2019年 / 11卷 / 01期
关键词：
Eudragit; Nutriose; phospholipid vesicles; antioxidant; intestinal wound healing; VESICLES;
D O I：
10.3390/pharmaceutics11010013
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
The present study aimed at developing a new vesicular formulation capable of promoting the protective effect of ascorbic acid and tocopherol against intestinal oxidative stress damage, and their efficacy in intestinal wound healing upon oral administration. A pH-dependent copolymer (Eudragit (R) L100), a water-soluble prebiotic fibre (Nutriose (R) FM06), a phospholipid mixture (Lipoid S75), and two natural antioxidants (ascorbic acid and tocopherol) were combined to fabricate eudragit-nutriosomes by a simple, solvent-free procedure. The vesicles were spherical and oligolamellar, with some multicompartment structures in Eudragit-nutriosomes, small in size (similar to 100 nm), with highly negative zeta potential. The effect of Eudragit (R) and Nutriose (R) on the stability on storage and in simulated gastrointestinal fluids were confirmed by the Turbiscan (R) technology and in vitro studies, respectively. Eudragit-nutriosomes exhibited a protective effect against H2O2-induced oxidative stress, and a proliferative effect in Caco-2 cells, as they provided the closure of the scratched area after 96 h of incubation.
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页数：10
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