The role of oxidative stress in acrolein-induced DNA damage in HepG2 cells

被引：49
|
作者：
Li, Longjie [1 ]
Jiang, Liping
Geng, Chengyan [2 ]
Cao, Jun [1 ]
Zhong, Laifu [1 ]
机构：
[1] Dalian Med Univ, Dept Toxicol, Dalian 116044, Liaoning, Peoples R China
[2] Dalian Med Univ, China Japanese Joint Inst Med & Pharmaceut Sci, Dalian 116044, Liaoning, Peoples R China
来源：
FREE RADICAL RESEARCH | 2008年 / 42卷 / 04期
基金：
中国国家自然科学基金;
关键词：
acrolein; single cell gel electrophoresis; DNA-protein crosslinks; oxidative stress; HepG2; cells;
D O I：
10.1080/10715760802008114
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
This study evaluated the role of oxidative stress in acrolein-induced DNA damage, using HepG2 cells. Using the standard single cell gel electrophoresis (SCGE) assay, a significant dose-dependent increment in DNA migration was detected at lower concentrations of acrolein; but at the higher tested concentrations, a reduction in the migration was observed. Post-incubation with proteinase K significantly increased DNA migration in cells exposed to higher concentrations of acrolein. These results indicated that acrolein caused DNA strand breaks and DNA-protein crosslinks (DPC). To elucidate the oxidatively generated DNA damage mechanism, the 2,7-dichlorofluorescein diacetate (DCFH-DA) and o-phthalaldehyde (OPT) were used to monitor the levels of reactive oxygen species (ROS) and glutathione (GSH), respectively. The present study showed that acrolein induced the increased levels of ROS and depletion of GSH in HepG2 cells. Moreover, acrolein significantly caused 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) formation in HepG2 cells. These results demonstrate that the DNA damage induced by acrolein in HepG2 cells is related to the oxidative stress.
引用
收藏
页码：354 / 361
页数：8
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