Detection of GB virus-C hepatitis G virus RNA in serum by reverse transcription polymerase chain reaction

被引：0
|
作者：
Bhardwaj, B
Qian, KP
Detmer, J
Mizokami, M
Kolberg, JA
Urdea, MS
Schlauder, G
Linnen, JM
Kim, JP
Davis, GL
Lau, JYN
机构：
[1] UNIV FLORIDA,DEPT MED,SECT HEPATOBILIARY DIS,DIV GASTROENTEROL HEPATOL & NUTR,GAINESVILLE,FL 32610
[2] CHIRON CORP,EMERYVILLE,CA 94608
[3] NAGOYA CITY UNIV,DEPT MED 2,NAGOYA,AICHI,JAPAN
[4] ABBOTT LABS,VIRUS DISCOVERY GRP,EXPT BIOL RES,CHICAGO,IL
[5] GENELABS TECHNOL INC,REDWOOD CITY,CA
来源：
JOURNAL OF MEDICAL VIROLOGY | 1997年 / 52卷 / 01期
关键词：
GBV-C; HGV; HCV; RNA; PCR; hybridization; serum; sensitivity; specificity;
D O I：
10.1002/(SICI)1096-9071(199705)52:1<92::AID-JMV15>3.0.CO;2-2
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Three PCR methods based on the GB virus-C/hepatitis G virus (GBV-C/HGV) 5'UTR and NS3 genomic region were used for the detection of GBV-C/HGV RNA in serum of 62 patients with chronic hepatitis C virus (HCV) infection. Ten of 62 (16%) patients were found to have GBV-C/HGV RNA, which was confirmed by sequence analysis of the 5'UTR PCR amplicon. All methods appear to be specific, but methods based on the 5'UTR appear to be more sensitive. (C) 1997 Wiley-Liss, Inc.
引用
收藏
页码：92 / 96
页数：5
相关论文
共 50 条
  • [31] Molecular evolutionary classification of GB virus-C hepatitis G virus.
    Ohba, K
    Mizokami, M
    Mukaide, M
    Orito, E
    Nakano, T
    Kato, T
    Kondo, Y
    Tanaka, Y
    Ueda, R
    Gojobori, T
    Lau, JYN
    HEPATOLOGY, 1997, 26 (04) : 1783 - 1783
  • [32] Effects of GB virus-C/hepatitis G virus on hepatitis B and C viremia in multiple hepatitis virus infections
    J.-H. Kao
    P.-J. Chen
    M.-Y. Lai
    W. Chen
    D.-S. Chen
    Archives of Virology, 1998, 143 : 797 - 802
  • [33] Specific detection of minus-strand hepatitis C virus RNA by reverse-transcription polymerase chain reaction on PolyA+-purified RNA
    Takyar, ST
    Li, DS
    Wang, YH
    Trowbridge, R
    Gowans, EJ
    HEPATOLOGY, 2000, 32 (02) : 382 - 387
  • [34] Prevalence of GB Virus-C Hepatitis G virus infection in an antenatal population
    Skidmore, SJ
    Collingham, KE
    JOURNAL OF MEDICAL VIROLOGY, 1999, 57 (03) : 235 - 237
  • [35] Potato virus a detection by reverse transcription-polymerase chain reaction
    Cerovská, N
    Petrzik, K
    Moravec, T
    Mráz, I
    ACTA VIROLOGICA, 1998, 42 (02) : 83 - 85
  • [36] Detection of Japanese encephalitis virus by reverse transcription/polymerase chain reaction
    Paranjpe, S
    Banerjee, K
    ACTA VIROLOGICA, 1998, 42 (01) : 5 - 11
  • [37] Integrated reverse transcription polymerase chain reaction systems for virus detection
    Lien, Kang-Yi
    Lee, Wan-Chi
    Lei, Huan-Yao
    Lee, Gwo-Bin
    BIOSENSORS & BIOELECTRONICS, 2007, 22 (08): : 1739 - 1748
  • [38] Detection of enteroviruses and hepatitis A virus RNA in milk by reverse transcriptase-polymerase chain reaction
    Terzi, G.
    Albayrak, H.
    Siriken, B.
    Cadirci, O.
    Yazici, Z.
    FEBS JOURNAL, 2009, 276 : 122 - 122
  • [39] Detection of hepatitis G virus (GB virus C) RNA in human saliva
    Chen, M
    Sonnerborg, A
    Johansson, B
    Sallberg, M
    JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (04) : 973 - 975
  • [40] Diagnostic Validation of the RealStar® Zika Virus Reverse Transcription Polymerase Chain Reaction Kit for Detection of Zika Virus RNA in Urine and Serum Specimens
    Oelschlaeger, Stephan
    Enfissi, Antoine
    Zaruba, Mareen
    Kazanji, Mirdad
    Rousset, Dominique
    AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2017, 97 (04): : 1070 - 1071
12345