Study on the Effects of Melatonin on Cisplatin-Induced Apoptosis of Renal Tubular Epithelial Cells and Autophagy Protein L Light Chain 3 (LC3)

Purpose: The aim of this work was to discuss Melatonin's effects on Cisplatin-induced Apoptosis of Renal Tubular Epithelial Cells. Materials and Methods: Blood and renal tissue were collected, The level of serum creatinine (Scr) was detected, the content of p53 protein in renal tissue was detected by immunohistochemistry, the expression of p53 protein and autophagy marker protein microtubule associated protein L light chain 3 broken vertical bar broken vertical bar (LC3 broken vertical bar broken vertical bar)/LC 3 broken vertical bar ratio in renal tissue was detected by WB method, the apoptosis of renal tubular epithelial cells was detected by TUNEL method, and the morphological changes of renal tissue were observed by light microscope. Results: Scr level and apoptosis cell rate were significantly increased in Model group (P < 0.001), P53 and LC 3 broken vertical bar broken vertical bar/LC 3I ratio were significantly depressed (P < 0.001). With MT supplement, Scr level and apoptosis cell rate were significantly depressed (P < 0.05); MT could significantly up-regulate P53 and LC 3 broken vertical bar broken vertical bar/LC 3 broken vertical bar ratio (P < 0.05), MT could improve the pathological changes such as renal tubular epithelial cell fusion and vacuolar degeneration, and reduce renal tubular injury. Conclusion: MT has a protective effect on cisplatin induced AKI in mice. Its mechanism may be related to up regulating the expression levels of p53 protein and LC 3 broken vertical bar broken vertical bar/LC 3 broken vertical bar ratio.