Transcriptome Modifications in the Porcine Intramuscular Adipocytes during Differentiation and Exogenous Stimulation with TNF-α and Serotonin

被引:17
|
作者
Tada, Asuka [1 ,2 ]
Islam, Md Aminul [1 ,2 ,3 ]
Kober, A. K. M. Humayun [1 ,2 ]
Fukuyama, Kohtaro [1 ,2 ]
Takagi, Michihiro [1 ,2 ]
Igata, Manami [1 ,2 ]
Albarracin, Leonardo [1 ,4 ]
Ikeda-Ohtsubo, Wakako [1 ,2 ]
Miyazawa, Kenji [5 ]
Yoda, Kazutoyo [5 ]
He, Fang [5 ]
Takahashi, Hideki [6 ,7 ]
Villena, Julio [1 ,4 ]
Aso, Hisashi [2 ,8 ]
Kitazawa, Haruki [1 ,2 ]
机构
[1] Tohoku Univ, Food & Feed Immunol Grp, Lab Anim Prod Chem, Grad Sch Agr Sci, Sendai, Miyagi 9818555, Japan
[2] Tohoku Univ, Grad Sch Agr Sci, Int Educ & Res Ctr Food Agr Immunol CFAI, Livestock Immunol Unit, Sendai, Miyagi 9818555, Japan
[3] Bangladesh Agr Univ, Dept Med, Fac Vet Sci, Mymensingh 2202, Bangladesh
[4] Consejo Nacl Invest Cient & Tecn, CERELA, Reference Ctr Lactobacilli, Lab Immunobiotechnol, RA-4000 San Miguel De Tucuman, Argentina
[5] Takanashi Milk Prod Co Ltd, Tech Res Lab, Yokohama, Kanagawa 2410023, Japan
[6] Tohoku Univ, Grad Sch Agr Sci, Lab Plant Pathol, Sendai, Miyagi 9818555, Japan
[7] Tohoku Univ, Grad Sch Agr Sci, Int Educ & Res Ctr Food Agr Immunol CFAI, Plant Immunol Unit, Sendai, Miyagi 9818555, Japan
[8] Tohoku Univ, Cell Biol Lab, Grad Sch Agr Sci, Sendai, Miyagi 9818555, Japan
基金
日本学术振兴会;
关键词
porcine intramuscular preadipocyte (PIP); porcine mature adipocyte (pMA); serotonin; serotonin receptor; adipogenesis; TNF-alpha; transcriptome; PROTEIN-COUPLED RECEPTORS; NECROSIS-FACTOR-ALPHA; NF-KAPPA-B; ADIPOSE-TISSUE; PERIPHERAL SEROTONIN; IN-VITRO; EXPRESSION; OBESITY; INFLAMMATION; PROMOTES;
D O I
10.3390/ijms21020638
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Adipocytes are dynamic cells that have critical functions to maintain body energy homeostasis. Adipocyte physiology is affected by the adipogenic differentiation, cell program, as well as by the exogenous stimulation of biochemical factors, such as serotonin and TNF-alpha. In this work, we investigated the global transcriptome modifications when porcine intramuscular preadipocyte (PIP) was differentiated into porcine mature adipocyte (pMA). Moreover, we studied transcriptome changes in pMA after stimulation with serotonin or TNF-alpha by using a microarray approach. Transcriptome analysis revealed that the expression of 270, 261, and 249 genes were modified after differentiation, or after serotonin and TNF-alpha stimulation, respectively. Expression changes in APP, HNF4A, ESR1, EGR1, SRC, HNF1A, FN1, ALB, STAT3, CBL, CEBPB, AR, FOS, CFTR, PAN2, PTPN6, VDR, PPARG, STAT5A and NCOA3 genes which are enriched in the 'PPAR signaling' and 'insulin resistance' pathways were found in adipocytes during the differentiation process. Dose-dependent serotonin stimulation resulted in a decreased fat accumulation in pMAs. Serotonin-induced differentially expressed genes in pMAs were found to be involved in the significant enrichment of ' GPCR ligand-binding ', 'cell chemotaxis', 'blood coagulation and complement', 'metabolism of lipid and lipoproteins', 'regulation of lipid metabolism by PPARA', and 'lipid digestion, mobilization and transport' pathways. TNF-alpha stimulation also resulted in transcriptome modifications linked with proinflammatory responses in the pMA of intramuscular origin. Our results provide a landscape of transcriptome modifications and their linked-biological pathways in response to adipogenesis, and exogenous stimulation of serotonin- and TNF-alpha to the pMA of intramuscular origin.
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页数:20
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