Changes in physiology and protein abundance in salt-stressed wheat chloroplasts

被引:91
|
作者
Kamal, Abu Hena Mostafa [1 ]
Cho, Kun [2 ]
Kim, Da-Eun [1 ]
Uozumi, Nobuyuki [3 ]
Chung, Keun-Yook [4 ]
Lee, Sang Young [5 ]
Choi, Jong-Soon [6 ,7 ]
Cho, Seong-Woo [8 ]
Shin, Chang-Seob [9 ]
Woo, Sun Hee [1 ]
机构
[1] Chungbuk Natl Univ, Dept Crop Sci, Cheongju 361763, Chungbuk, South Korea
[2] Korea Basic Sci Inst, Div Mass Spectrometry Res, Ochang 863883, Chungbuk, South Korea
[3] Tohoku Univ, Grad Sch Engn, Dept Biomol Engn, Sendai, Miyagi 9808579, Japan
[4] Chungbuk Natl Univ, Dept Agr Chem, Cheongju 361763, Chungbuk, South Korea
[5] Watermelon Res Inst, Chungbuk Prov Agr Res Inst, Eumseong 369820, South Korea
[6] Korea Basic Sci Inst, Div Life Sci, Taejon 305333, South Korea
[7] Chungnam Natl Univ, Grad Sch Analyt Sci & Technol, Taejon 305764, South Korea
[8] Tottori Univ, Arid Land Res Ctr, Lab Mol Breeding, Yonago, Tottori 6800001, Japan
[9] Chungbuk Natl Univ, Dept Forest Sci, Cheongju 361763, Chungbuk, South Korea
关键词
Chloroplast; Salt stress; LTQ-FT system; Proteomics; Wheat; PROTEOMIC ANALYSIS; HYDROGEN-PEROXIDE; 2-DIMENSIONAL ELECTROPHORESIS; SUPEROXIDE-DISMUTASE; RESPONSIVE PROTEINS; TRITICUM-AESTIVUM; MESSENGER-RNA; PROLINE ACCUMULATION; MORINDA CITRIFOLIA; ADVENTITIOUS ROOTS;
D O I
10.1007/s11033-012-1777-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Leaves are the final site of salinity perception through the roots. To better understand how wheat chloroplasts proteins respond to salt stress, the study aimed to the physiochemical and comparative proteomics analysis. Seedlings (12-days-old) were exposed to 150 mM NaCl for 1, 2, or 3 days. Na+ ions were rapid and excessively increase in roots, stems and leaves. Photosynthesis and transpiration rate, stomatal conductance, and relative water content decreased whereas the level of proline increased. Statistically significant positive correlations were found among the content of hydrogen peroxide, activity of catalase, and superoxide dismutase under salt stress in wheat. Protein abundance within the chloroplasts was examined by two-dimensional electrophoresis. More than 100 protein spots were reproducibly detected on each gel, 21 protein spots were differentially expressed during salt treatment. Using linear quadruple trap-Fourier transform ion cyclotron resonance (LTQ-FTICR) hybrid mass spectrometry, 65 unique proteins assigned in the differentially abundant spots. Most proteins were up-regulated at 2 and 3 days after being down-regulated at 1 day. Others showed only slight responses after 3 days of treatment, including Rubisco, glutamate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, isocitrate dehydrogenase, photosystem I, and pyridoxal biosynthesis protein PDX1.2 and PDX1.3. The ATP synthase (alpha, beta, and gamma) and V-type proton ATPase subunits were down-regulated resulting showed negative impact by Na+ on the photosynthetic machinery. This ephemeral increase and subsequent decrease in protein contents may demonstrate a counterbalancing influence of identified proteins. Several proteins such as cytochrome b6-f (Cyt b6-f), germin-like-protein, the gamma-subunit of ATP synthase, glutamine synthetase, fructose-bisphosphate aldolase, S-adenosylmethionine synthase, carbonic anhydrase were gradually up-regulated during the period of treatment, which can be identified as marker proteins.
引用
收藏
页码:9059 / 9074
页数:16
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