Immobilization of Invertase on Chitosan and Its Application to Honey Treatment

被引：1

作者：

Nguyen Xuan Nam ^{[1
]}

Ho Trung Trong Nghia ^{[1
]}

Le Thi Tuong Vy ^{[1
]}

Huynh Ngoc Oanh ^{[1
]}

Phan Phuoc Hien ^{[2
]}

机构：

[1] Ho Chi Minh City Univ Technol, Ho Chi Minh City, Vietnam

[2] Southern Cantho Univ, Can Tho, Vietnam

来源：

INTERNATIONAL CONFERENCE ON CHEMICAL ENGINEERING, FOOD AND BIOTECHNOLOGY (ICCFB2017) | 2017年 / 1878卷

关键词：

ANTIOXIDANT ACTIVITY; CHITIN; POLYACRYLAMIDE; HYDROLYSIS; ENZYMES; COLOR;

D O I：

10.1063/1.5000173

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The optimal conditions for immobilized enzyme invertase on chitosan were studied. Beside that, the aim of the present work was to find out if the processing with chitosan invertase can affect some of the main honey quality paramenters - reductive sugar (RS), colour and antioxidant activity. RS content were analyzed by DNS method, colour parameters (L*, a*, b*) were established in the CIE system and antioxidant activity were analyzed by DPPH method. The results showed that the immobilized conditions were as follows: ratio chitosan/invertase 7/1 (w/w), invertase 0.8%, glutaraldehyde 4%, 50 degrees C at 60 minutes, pH 4.5. After the treatments, there was significant (P<0.05) difference on RS (58.38% for melaleuca honey, 15.11% for dimocarpus longan honey) and colour (Delta E=3.84 for melaleuca honey and Delta E =2.76 for dimocarpus longan honey). Moreover, there was no significant (P<0.05) difference on antioxidant activity.

引用

页数：9

共 50 条

[41] Application of Hierarchically Porous Chitosan Monolith for Enzyme Immobilization
Hajili, Emil
Sugawara, Akihide
Uyama, Hiroshi
BIOMACROMOLECULES, 2024, 25 (06) : 3486 - 3498
[42] IMMOBILIZATION OF CELLULASES ON CHITOSAN: APPLICATION FOR SUGARCANE BAGASSE HYDROLYSIS
Pereira, Mariana Bisinotto
Nogueira, Bruno Lemos
Cavalcanti Montano, Inti Doraci
Rodrigues, Dasciana De Sousa
Galeano Suarez, Carlos Alberto
CELLULOSE CHEMISTRY AND TECHNOLOGY, 2021, 55 (7-8): : 829 - 837
[43] APPLICATION OF CHITOSAN AS SUPPORT FOR IMMOBILIZATION OF ENZYMES OF INDUSTRIAL INTEREST
Mendes, Adriano A.
de Oliveira, Pedro C.
de Castro, Heizir F.
Giordano, Raquel de L. C.
QUIMICA NOVA, 2011, 34 (05): : 831 - 840
[44] Clinoptilolite particles as a carrier for biocatalysts immobilization: invertase immobilization and characterization
Bahar, Tahsin
ASIA-PACIFIC JOURNAL OF CHEMICAL ENGINEERING, 2014, 9 (01) : 31 - 38
[45] IMMOBILIZATION OF YEAST BY INVERTASE ADSORPTION ON MAGNETITE
KUBAL, BS
SANKARAN, K
DSOUZA, SF
JOURNAL OF MICROBIAL BIOTECHNOLOGY, 1989, 4 (02): : 98 - 102
[46] IMMOBILIZATION OF INVERTASE BY ENCAPSULATION IN POLYELECTROLYTE COMPLEXES
MANSFELD, J
FORSTER, M
SCHELLENBERGER, A
DAUTZENBERG, H
ENZYME AND MICROBIAL TECHNOLOGY, 1991, 13 (03) : 240 - 244
[47] Immobilization of pectinase on oxidized pulp fiber and its application in whitewater treatment
Wu, Rina
He, Bei-Hai
Zhao, Guang-Lei
Qian, Li-Ying
Li, Xiao-Feng
CARBOHYDRATE POLYMERS, 2013, 97 (02) : 523 - 529
[48] IMMOBILIZATION OF NITRIFYING BACTERIA AND ITS APPLICATION FOR WASTE-WATER TREATMENT
ITOH, K
ITADANI, T
YOSIMURA, H
SHINODA, S
EISEI KAGAKU-JAPANESE JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH, 1989, 35 (02): : 125 - 133
[49] Immobilization of chitosan-modified invertase on alginate-coated chitin support via polyelectrolyte complex formation
Gómez, L
Ramírez, HL
Villalonga, ML
Hernández, J
Villalonga, R
ENZYME AND MICROBIAL TECHNOLOGY, 2006, 38 (1-2) : 22 - 27
[50] Immobilization of invertase on chitosan coated γ-Fe2O3 magnetic nanoparticles to facilitate magnetic separation
Waifalkar, P. P.
Parit, S. B.
Chougale, A. D.
Sahoo, Subasa C.
Patil, P. S.
Patil, P. B.
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 2016, 482 : 159 - 164

← 1 2 3 4 5 →