Minicircle DNA-Engineered CAR T Cells Suppressed Tumor Growth in Mice

被引：13

作者：

Han, Jinsheng ^{[1
]}

Gao, Fei ^{[1
,2
]}

Geng, Songsong ^{[4
]}

Ye, Xueshuai ^{[1
]}

Wang, Tie ^{[3
,4
]}

Du, Pingping ^{[4
]}

Cai, Ziqi ^{[4
]}

Fu, Zexian ^{[5
]}

Zhao, Zhilong ^{[6
]}

Shi, Long ^{[7
]}

Li, Qingxia ^{[2
]}

Cai, Jianhui ^{[1
,2
]}

机构：

[1] Hebei Med Univ, Dept Surg, Shijiazhuang, Hebei, Peoples R China

[2] Hebei Gen Hosp, Dept Surg & Oncol, Shijiazhuang, Hebei, Peoples R China

[3] Hebei HOFOY Biotech Co Ltd, Hebei Engn Technol Res Ctr Cell Therapy, Shijiazhuang, Hebei, Peoples R China

[4] Hebei Cangzhou Hosp Integrated Tradit Chinese & W, Dept Surg, Cangzhou, Hebei, Peoples R China

[5] Hebei Engn Univ, Dept Surg, Affiliated Hosp, Handan, Hebei, Peoples R China

[6] Jinzhou Med Univ, Dept Surg, Affiliated Hosp 3, Jinzhou, Liaoning, Peoples R China

[7] Hebei Med Univ, Dept Oncol, Hosp 2, Shijiazhuang, Hebei, Peoples R China

来源：

MOLECULAR CANCER THERAPEUTICS | 2020年 / 19卷 / 01期

关键词：

CHIMERIC ANTIGEN RECEPTOR; EXPRESSION; VECTOR; TRANSPOSON; THERAPY;

D O I：

10.1158/1535-7163.MCT-19-0204

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Viral-based chimeric antigen receptor-engineered T (CAR T)-cell manufacturing has potential safety risks and relatively high costs. The nonviral minicircle DNA (mcDNA) is safer for patients, cheaper to produce, and may be a more suitable technique to generate CAR T cells. In this study, we produced mcDNA-based CAR T cells specifically targeting prostate stem cell antigen (PSCA; mcDNA-PSCA-CAR T cells). Our results showed that mcDNA-PSCA-CAR T cells persisted in mouse peripheral blood as long as 28 days and demonstrated more CAR T-cell infiltration, higher cytokine secretion levels, and better antitumor effects. Together, our results suggest that mcDNA-CAR can be a safe and cost-effective platform to produce CAR T cells.

引用

页码：178 / 186

页数：9

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