The distribution of human leukocyte antigen-A, -B, and -DRB1 alleles and haplotypes based on high-resolution genotyping of 167 families from Jiangsu Province, China

We investigated the human leukocyte antigen (HLA)-A, -B, and -DRB1 allele frequencies, the A-B-DRB1, A-B, B-DRB1, and A-DRB1 haplotype frequencies, and the characteristics of linkage disequilibrium between 2 loci in high resolution based on 167 unrelated families from Jiangsu Province, China. A total of 26 alleles at the A locus, 55 alleles at the B locus, and 34 alleles at the DRB1 locus were reported in this study. The top 5 most frequent HLA alleles at the HLA-A, -B, and -DRB1 loci, respectively, were A*11:01, A*24:02, A*02:01, A*33:03, A*30:01; B*13:02, B*40:01 B*46:01, B*58:01, B*54:01; DRB1*09:01, DRB1*07:01, DRB1*12:02, DRB1*15:01, and DRB1*08:03. Several haplotypes with high frequencies were deduced in this study. The top 3 most common A-B-DRB1 haplotypes observed were A*30:01-B*13:02-DRB1*07:01, A*33:03-B*58:01-DRB1*03: 01, and A*02:07-B*46:01-DRB1*09:01. The top 3 most common A-B haplotypes were A*30:01-B*13:02, A*33:03-B*58:01, and A*02:07-B*46:01. The top 4 most common A-DRB1 haplotypes were A*30:01-DRB1*07:01, A*33:03-DRB1*13:02, A*24:02-DRB1*09:01, and A*33:03-DRB1*03:01. Finally, the top 3 most common B-DRB1 haplotypes were B*13:02-DRB1*07:01, B*46:01-DRB1*09:01, and B*58:01-DRB1*03:01. From the linkage disequilibrium calculation, the most prominent associations were A*30:01-B*13:02. B*13: 02-DRB1*07:01, and A*01:03-DRB1*01:02. These allele and haplotype frequencies could be useful for finding the best matched donors for patients in the China Marrow Donor Program Jiangsu Branch. (C) 2011 American Society for Histocompatibility and Immunogenetics. Published by Elsevier Inc. All rights reserved.