Comparison of several mathematical models for describing the joint effect of temperature and ph on glucanex activity

被引：21

作者：

Prieto, Miguel Angel ^{[1
]}

Vazquez, Jose Antonio ^{[1
]}

Murado, Miguel Anxo ^{[1
]}

机构：

[1] CSIC, Inst Invest Marinas, Grp Reciclado & Valorizac Mat Residuais REVAL, Vigo 36208, Galicia, Spain

来源：

BIOTECHNOLOGY PROGRESS | 2012年 / 28卷 / 02期

关键词：

glucanex; enzymatic activity; curdlan; laminarin; mathematical modeling; pH and temperature effects; SOLUBLE BETA-GLUCAN; CELL-WALL; REACTION-KINETICS; CROSS-VALIDATION; GROWTH-RATE; SELECTION; ENZYMES; HYDROLYSIS; REGRESSION; STABILITY;

D O I：

10.1002/btpr.733

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The aim of the present work was to evaluate with different statistical criteria the suitability of nine equations for describing and optimizing the simultaneous effect of temperature and pH on glucanex activity using two characteristic polysaccharides (curdlan and laminarin) as substrates. The most satisfactory solutions were found with an empirical equation constituted with parameters of practical interest (Rosso model), and a hybrid model between the Arrhenius equation and the mathematical expression generated by the protonation-hydroxylation mechanism (Tijskens model). The joint optimal values of pH and temperature calculated with the Rosso model were obtained at 4.64 and 50 degrees C with curdlan and 4.64 and 48 degrees C using laminarin as substrate. (c) 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2012

引用

页码：372 / 381

页数：10

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