Development and performance of prototype serologic and molecular tests for hepatitis delta infection

被引:15
|
作者
Coller, Kelly E. [1 ]
Butler, Emily K. [1 ]
Luk, Ka-Cheung [1 ]
Rodgers, Mary A. [1 ]
Cassidy, Michael [1 ]
Gersch, Jeffrey [1 ]
McNamara, Anne L. [1 ]
Kuhns, Mary C. [1 ]
Dawson, George J. [1 ]
Kaptue, Lazare [2 ]
Bremer, Birgit [3 ]
Wedemeyer, Heiner [3 ]
Cloherty, Gavin A. [1 ]
机构
[1] Abbott Labs, Abbott Pk, IL 60064 USA
[2] Univ Montagnes, Bangangte, Cameroon
[3] Hannover Med Sch, Dept Gastroenterol Hepatol & Endocrinol, Hannover, Germany
来源
SCIENTIFIC REPORTS | 2018年 / 8卷
基金
美国国家卫生研究院;
关键词
D-VIRUS; B-VIRUS; GENETIC-VARIABILITY; GENOTYPE; RNA; QUANTITATION; QUANTIFICATION; TRANSMISSION; ANTIGEN; ASSAY;
D O I
10.1038/s41598-018-20455-5
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Worldwide, an estimated 5% of hepatitis B virus (HBV) infected people are coinfected with hepatitis delta virus (HDV). HDV infection leads to increased mortality over HBV mono-infection, yet HDV diagnostics are not widely available. Prototype molecular (RNA) and serologic (IgG) assays were developed for high-throughput testing on the Abbott m2000 and ARCHITECT systems, respectively. RNA detection was achieved through amplification of a ribozyme region target, with a limit of detection of 5 IU/ml. The prototype serology assay (IgG) was developed using peptides derived from HDV large antigen (HDAg), and linear epitopes were further identified by peptide scan. Specificity of an HBV negative population was 100% for both assays. A panel of 145 HBsAg positive samples from Cameroon with unknown HDV status was tested using both assays: 16 (11.0%) had detectable HDV RNA, and 23 (15.7%) were sero-positive including the 16 HDV RNA positive samples. Additionally, an archival serial bleed panel from an HDV superinfected chimpanzee was tested with both prototypes; data was consistent with historic testing data using a commercial total anti-Delta test. Overall, the two prototype assays provide sensitive and specific methods for HDV detection using high throughput automated platforms, allowing opportunity for improved diagnosis of HDV infected patients.
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页数:11
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