Production, preliminary characterization and antitumor activity in vitro of polysaccharides from the mycelium of Pholiota dinghuensis Bi

被引：106
|
作者：
Gan, Dan [1 ,2 ]
Ma, Liping [1 ]
Jiang, Changxing [1 ]
Xu, Renjie [1 ]
Zeng, Xiaoxiong [1 ]
机构：
[1] Nanjing Agr Univ, Coll Food Sci & Technol, Nanjing 210095, Peoples R China
[2] Xuzhou Coll Ind Technol, Dept Chem Engn, Xuzhou 221140, Peoples R China
来源：
CARBOHYDRATE POLYMERS | 2011年 / 84卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Pholiota dinghuensis Bi; Polysaccharide; Production; Preliminary characterization; Antitumor activity; SUBMERGED CULTURE-CONDITIONS; GRIFOLA-FRONDOSA; OPTIMIZATION; EXOPOLYSACCHARIDES; PURIFICATION; EXTRACTION; NAMEKO;
D O I：
10.1016/j.carbpol.2010.12.058
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
The production, preliminary characterization and antitumor activity in vitro of mycelial polysaccharides from Pholiota dinghuensis Bi (PDP) were investigated in the present study. Firstly, crude PDP was prepared from the mycelia of P. dinghuensis Bi by submerged culture. Then, the crude PDP was purified by chromatography of DEAE-52 cellulose and Sephadex G-100, resulting three purified fractions of PDP-1. PDP-2 and PDP-3. We found that the monosaccharide composition of PDP-3 was greatly different from that of PDP-1 or PDP-2. In addition, it contained the highest contents of protein, sulfate and uronic acid among the polysaccharides tested. Furthermore, PDP-3 exhibited higher antiproliferative activity against human gastric cancer BGC-823 cells in vitro than crude PDP. PDP-1 or PDP-2. At a concentration of 400 mg/L and an exposure time of 72 h, the inhibition rates for crude PDP, PDP-2 and PDP-3 were 69.36%, 73.65% and 85.78%, respectively. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：997 / 1003
页数：7
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