Clinical evaluation of a panel of multiplex quantitative real-time reverse transcription polymerase chain reaction assays for the detection of 16 respiratory viruses associated with community-acquired pneumonia

被引：11
|
作者：
Zhang, Dan [1 ,2 ]
Mao, Haiyan [2 ]
Lou, Xiuyu [2 ]
Pan, Junhang [2 ]
Yan, Hao [2 ]
Tang, Hongfeng [3 ]
Shu, Yan [3 ]
Zhao, Yun [3 ]
Cheng, Xiaoli [4 ]
Tao, Hong [5 ]
Zhang, Yanjun [2 ]
Ma, Xuejun [1 ]
机构：
[1] Chinese Ctr Dis Control & Prevent, Natl Inst Viral Dis Control & Prevent, Key Lab Med Virol, Natl Hlth & Family Planning Commiss, 155 Changbai Rd, Beijing 102206, Peoples R China
[2] Zhejiang Prov Ctr Dis Control & Prevent, Inst Microbiol, Hangzhou 310051, Zhejiang, Peoples R China
[3] Zhejiang Univ, Childrens Hosp, Dept Pathol, Sch Med, Hangzhou 310013, Zhejiang, Peoples R China
[4] Hubei Univ Med, Sch Basic Med, Dept Pathol, Shiyan 442000, Hubei, Peoples R China
[5] Hubei Univ Med, Renmin Hosp, Dept Rheumatol & Immunol, Shiyan 442000, Hubei, Peoples R China
来源：
ARCHIVES OF VIROLOGY | 2018年 / 163卷 / 10期
关键词：
EPIDEMIOLOGY; INFECTIONS; SPECIMENS;
D O I：
10.1007/s00705-018-3921-8
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We developed a panel of multiplex quantitative real-time reverse transcription polymerase chain reaction (mqRT-PCR) assay consisting of seven internally controlled qRT-PCR assays to detect 16 different respiratory viruses. We compared the new mqRT-PCR with a previously reported two-tube mRT-PCR assay using 363 clinical sputum specimens. The mqRT-PCR assay performed comparably with the two-tube assay for most viruses, offering the advantages of quantitative analysis, easier performance, lower susceptibility to contamination, and shorter turnaround time in laboratories equipped with conventional real-time PCR instrumentation, and it could therefore be a valuable tool for routine surveillance of respiratory virus infections in China.
引用
收藏
页码：2855 / 2860
页数：6
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