Antimicrobial susceptibility of Mycoplasma bovis isolates from Bosnia and Herzegovina

The minimum inhibitory concentrations (MICs) of enrofloxacin, ciprofloxacin, norfloxacin, gentamicin, spectinomycin, oxytetracycline, tylosin, florfenicol and tiamulin were determined against 24 Mycoplasma bovis isolates from cattle in Bosnia and Herzegovina using the microbroth dilution test. The lowest MIC values (<= 0.03 mu g/ml) were obtained for tiamulin, gentamicin and oxytetracycline. Spectinomycin and tylosin had the highest MIC values (>= 128 mu g/ml). Tiamulin had the lowest MIC50 value (<0.03 mu g/ml) and MIC90 value (0.5 mu g/ml). Among the fluoroquinolones, enrofloxacin had the lowest MIC50 value (0.25 mu g/ml) and MIC90 value (1 mu g/ml), followed by ciprofloxacin (MIC50 of 0.5 mu g/ml; MIC90 of 2 mu g/ml). The highest MIC50 value (4 mu g/ml) was found for norfloxacin, oxytetracycline and tylosin, while spectinomycin and tylosin yielded the highest MIC90 values (>128 mu g/ml).