Design and application of a microfluidic cell lysis microelectrode chip

被引：8
|
作者：
Jiang, Feng [1 ,2 ]
Chen, Jianjun [2 ,3 ]
Yu, Jun [2 ]
机构：
[1] Cent South Univ Forestry & Technol, Sch Comp & Informat Engn, Changsha 410004, Hunan, Peoples R China
[2] Huazhong Univ Sci & Technol, Sch Opt & Elect Informat, Wuhan 430074, Peoples R China
[3] Huazhong Agr Univ, Coll Sci, Wuhan, Peoples R China
来源：
INSTRUMENTATION SCIENCE & TECHNOLOGY | 2016年 / 44卷 / 02期
基金：
中国国家自然科学基金;
关键词：
Cell lysis; microelectrode chip; microfluidic; POST-GENOME ERA; DC VOLTAGE; DEVICE; ELECTROPORATION;
D O I：
10.1080/10739149.2015.1076839
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Microelectrode chips were designed and prepared for cell lysis. The method involved pulverization of the cells by an electrical signal and attachment on the chip. Cell lysis was performed by a 10 volt square wave. This is a reduced voltage compared with traditional methods. This design offers an improved lysing rate, simple operation and design, and low cost.
引用
收藏
页码：223 / 232
页数：10
相关论文
共 50 条
  • [31] Assessment of mitochondrial membrane potential using an on-chip microelectrode in a microfluidic device
    Lim, Tae-Sun
    Davila, Antonio
    Wallace, Douglas C.
    Burke, Peter
    LAB ON A CHIP, 2010, 10 (13) : 1683 - 1688
  • [32] Microfluidic chip with interdigitated ultra-microelectrode array for total phosphorus detection
    Bai, Yin
    Tong, Jianhua
    Sun, Jizhou
    Li, Yang
    Bian, Chao
    Xia, Shanhong
    MICRO & NANO LETTERS, 2014, 9 (12): : 862 - 865
  • [33] Control and application of microdroplet in microfluidic chip
    Kai, Zhang
    Ping, Hu
    Qiong-Lin, Liang
    Guo-An, Luo
    CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2008, 36 (04) : 556 - 562
  • [34] Fabrication of microfluidic chip and its application
    Du, LQ
    Liu, C
    Luo, Y
    Luo, ZF
    Chu, D
    Wen, M
    Chen, R
    PROCEEDINGS OF THE 2004 INTERNATIONAL SYMPOSIUM ON MICRO-NANOMECHATRONICS AND HUMAN SCIENCE, 2004, : 237 - 242
  • [35] Design of the microfluidic chip of oil detection
    Zhang, Hongpeng
    Huang, Wen
    Zhang, Yindong
    Shen, Yan
    Li, Dongqing
    MATERIALS AND COMPUTATIONAL MECHANICS, PTS 1-3, 2012, 117-119 : 517 - +
  • [36] Chitosan microfiber fabrication using a microfluidic chip and its application to cell cultures
    Yeh, Chia-Hsien
    Lin, Po-Wen
    Lin, Yu-Cheng
    MICROFLUIDICS AND NANOFLUIDICS, 2010, 8 (01) : 115 - 121
  • [37] Chitosan microfiber fabrication using a microfluidic chip and its application to cell cultures
    Chia-Hsien Yeh
    Po-Wen Lin
    Yu-Cheng Lin
    Microfluidics and Nanofluidics, 2010, 8 : 115 - 121
  • [38] Rapid Fabrication of Hydrogel Microfluidic Chip and Its Application for Cell Metabolites Detection
    Huo Dan-Qun
    Fang Ke-Jing
    Hou Chang-Jun
    Yang Mei
    Fa Huan-Bao
    Huang Cheng-Hong
    Luo Xiao-Gang
    CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE, 2013, 34 (06): : 1327 - 1332
  • [39] Continuous cell cross over and lysis in a microfluidic device
    Mun, Byung Phil
    Jung, Seung Mo
    Yoon, Sang Youl
    Kim, So Hee
    Lee, Jong Hyun
    Yang, Sung
    MICROFLUIDICS AND NANOFLUIDICS, 2010, 8 (05) : 695 - 701
  • [40] Continuous cell cross over and lysis in a microfluidic device
    Byung Phil Mun
    Seung Mo Jung
    Sang Youl Yoon
    So Hee Kim
    Jong Hyun Lee
    Sung Yang
    Microfluidics and Nanofluidics, 2010, 8 : 695 - 701
12345