A sensitive biosensor for the fluorescence detection of the acetylcholinesterase reaction system based on carbon dots

被引:32
|
作者
Ren, Xiangling [1 ,3 ]
Wei, Jianfei [1 ,2 ]
Ren, Jun [1 ]
Qiang, Li [1 ,2 ]
Tang, Fangqiong [1 ]
Meng, Xianwei [1 ]
机构
[1] Chinese Acad Sci, Tech Inst Phys & Chem, Lab Controllable Preparat & Applicat Nanomat, Beijing 100190, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[3] Southeast Univ, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
Carbon dots; Fluorescence; Biosensor; Hydrogen peroxide; Acetylcholinesterase; SEMICONDUCTOR QUANTUM DOTS; NANOCOMPOSITE FILMS; FENTON REACTION; NANODOTS; OXIDATION; PROBES;
D O I
10.1016/j.colsurfb.2014.11.007
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The carbon dots (C-dots) with high fluorescence quantum yield were prepared using hydrothermal method. C-dots have been adopted as probes for the fluorescence turn-off detection of H2O2 based on the special sensibility for the hydroxyl radical. And then the biosensors for the detection of substrate and enzymes activities were established in the acetylcholinesterase reaction system, which were related to the production of H2O2. Specifically, the proposed fluorescent biosensor was successfully applied to detect the concentration of choline (in the range from 0.025 to 50 mu M) and acetylcholine (in the range from 0.050 to 50 mu M), and the activity of choline oxidase (in the range from 1 to 75 U/L) and acetylcholinesterase (1 to 80 U/L). These results showed a sensitive, universal, nontoxic and eco-friendly detecting technique has been developed. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码:90 / 95
页数:6
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