Hydrolysis of DNA by a branched peptide without aromatic residues

被引：0

作者：

Xiao, AS

Feng, YP ^{[1
]}

Cao, SL

Xie, WJ

Zhao, WF

机构：

[1] Tsing Hua Univ, Minist Educ, Key Lab Bioorgan Phosphorus & Chem Biol, Beijing 100084, Peoples R China

[2] Capital Normal Univ, Dept Chem, Beijing 100037, Peoples R China

来源：

INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS | 2005年 / 11卷 / 03期

基金：

中国国家自然科学基金;

关键词：

branched peptide; double-strand DNA; hydrolysis;

D O I：

10.1007/s10989-005-6788-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A branched peptide N alpha, N epsilon-di (L-leucyl)-L-lysine was found to efficiently cleave supercoiled double-strand DNA such as PUC19 DNA at optimum pH 4.0 in 40 mmol/l Britton-Robbinson buffer. The T4 ligase experiment implied that the DNA cleavage occurs via a hydrolytic path. The dependence of the cleavage reaction on the ionic strength indicated that the interaction of DNA with the branched peptide involve only electrostatic binding.

引用

页码：181 / 183

页数：3

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