Hydrolysis of DNA by a branched peptide without aromatic residues

被引：0
|
作者：
Xiao, AS
Feng, YP [1 ]
Cao, SL
Xie, WJ
Zhao, WF
机构：
[1] Tsing Hua Univ, Minist Educ, Key Lab Bioorgan Phosphorus & Chem Biol, Beijing 100084, Peoples R China
[2] Capital Normal Univ, Dept Chem, Beijing 100037, Peoples R China
来源：
INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS | 2005年 / 11卷 / 03期
基金：
中国国家自然科学基金;
关键词：
branched peptide; double-strand DNA; hydrolysis;
D O I：
10.1007/s10989-005-6788-y
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A branched peptide N alpha, N epsilon-di (L-leucyl)-L-lysine was found to efficiently cleave supercoiled double-strand DNA such as PUC19 DNA at optimum pH 4.0 in 40 mmol/l Britton-Robbinson buffer. The T4 ligase experiment implied that the DNA cleavage occurs via a hydrolytic path. The dependence of the cleavage reaction on the ionic strength indicated that the interaction of DNA with the branched peptide involve only electrostatic binding.
引用
收藏
页码：181 / 183
页数：3
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