pDNA loaded calcium phosphate nanoparticles: highly efficient non-viral vector for gene delivery

被引：195
|
作者：
Bisht, S [1 ]
Bhakta, G [1 ]
Mitra, S [1 ]
Maitra, A [1 ]
机构：
[1] Univ Delhi, Dept Chem, Delhi 110007, India
来源：
INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2005年 / 288卷 / 01期
关键词：
calcium phosphate nanoparticles; non-viral vectors; gene delivery; transfection efficiency;
D O I：
10.1016/j.ijpharm.2004.07.035
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Nanoparticles of calcium phosphate encapsulating plasmid DNA (pDNA) of size 100-120nm in diameter were prepared. XRD studies of these nanoparticles showed them to be crystalline in nature having hydroxyapatite structure. The maximum loading of pDNA and its release from nanoparticles were studied using gel electrophoresis. The time dependent size measurement of these particles demonstrated that these particles show strong aggregational behaviour in aqueous dispersion. Calcium phosphate nanoparticles were found to be dissolved even in low acidic buffer (pH 5.0) releasing the pDNA, which suggested that DNA release from these particles in the endosomal compartment was possible. In vitro transfection efficiency of these calcium phosphate nanoparticles was found to be higher than that of the commercial transfecting reagent Polyfect. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：157 / 168
页数：12
相关论文
共 50 条
  • [21] Gemini nanoparticles as non-viral gene delivery systems
    Foldvari, M.
    Badea, I
    Wettig, S.
    Verrall, R.
    NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE, 2006, 2 (04) : 271 - 271
  • [22] Novel polyallylamine-dextran sulfate-DNA nanoplexes: Highly efficient non-viral vector for gene delivery
    Nimesh, Surendra
    Kumar, Rupesh
    Chandra, Ramesh
    INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2006, 320 (1-2) : 143 - 149
  • [23] Progress of cationic gene delivery reagents for non-viral vector
    Ma, Kai
    Mi, Chun-Liu
    Cao, Xiang-Xiang
    Wang, Tian-Yun
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2021, 105 (02) : 525 - 538
  • [24] Progress of cationic gene delivery reagents for non-viral vector
    Kai Ma
    Chun-Liu Mi
    Xiang-Xiang Cao
    Tian-Yun Wang
    Applied Microbiology and Biotechnology, 2021, 105 : 525 - 538
  • [25] Non-viral gene delivery
    Behr, JP
    EUROCANCER 98, 1998, : 255 - 256
  • [26] Hyaluronic acid hydrogel scaffolds loaded with cationic niosomes for efficient non-viral gene delivery
    Villate-Beitia, Ilia
    Truong, Norman F.
    Gallego, Idoia
    Zarate, Jon
    Puras, Gustavo
    Luis Pedraz, Jose
    Segura, Tatiana
    RSC ADVANCES, 2018, 8 (56): : 31934 - 31942
  • [27] DNA encapsulated magnesium and manganous phosphate nanoparticles: potential non-viral vectors for gene delivery
    Bhakta, G
    Mitra, S
    Maitra, A
    BIOMATERIALS, 2005, 26 (14) : 2157 - 2163
  • [28] Graphene-encapsulated iron nanoparticles as a non-viral vector for gene delivery into melanoma cells
    Bamburowicz-Klimkowska, Magdalena
    Malecki, Maciej
    Bystrzejewski, Michal
    Kasprzak, Artur
    Grudzinski, Ireneusz P.
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2023, 652 : 84 - 87
  • [29] ORMOSIL nanoparticles as a non-viral gene delivery vector for modeling polyglutamine induced brain pathology
    Klejbor, I.
    Stachowiak, E. K.
    Bharali, D. J.
    Roy, I.
    Spodnik, I.
    Morys, J.
    Bergey, E. J.
    Prasad, P. N.
    Stachowiak, M. K.
    JOURNAL OF NEUROSCIENCE METHODS, 2007, 165 (02) : 230 - 243
  • [30] Hyperbranched PEGmethacrylate linear pDMAEMA block copolymer as an efficient non-viral gene delivery vector
    Mathew, Asha
    Cao, Hongliang
    Collin, Estelle
    Wang, Wenxin
    Pandit, Abhay
    INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2012, 434 (1-2) : 99 - 105
12345