pDNA loaded calcium phosphate nanoparticles: highly efficient non-viral vector for gene delivery

被引：195
|
作者：
Bisht, S [1 ]
Bhakta, G [1 ]
Mitra, S [1 ]
Maitra, A [1 ]
机构：
[1] Univ Delhi, Dept Chem, Delhi 110007, India
来源：
INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2005年 / 288卷 / 01期
关键词：
calcium phosphate nanoparticles; non-viral vectors; gene delivery; transfection efficiency;
D O I：
10.1016/j.ijpharm.2004.07.035
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Nanoparticles of calcium phosphate encapsulating plasmid DNA (pDNA) of size 100-120nm in diameter were prepared. XRD studies of these nanoparticles showed them to be crystalline in nature having hydroxyapatite structure. The maximum loading of pDNA and its release from nanoparticles were studied using gel electrophoresis. The time dependent size measurement of these particles demonstrated that these particles show strong aggregational behaviour in aqueous dispersion. Calcium phosphate nanoparticles were found to be dissolved even in low acidic buffer (pH 5.0) releasing the pDNA, which suggested that DNA release from these particles in the endosomal compartment was possible. In vitro transfection efficiency of these calcium phosphate nanoparticles was found to be higher than that of the commercial transfecting reagent Polyfect. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：157 / 168
页数：12
相关论文
共 50 条
  • [1] Calcium phosphate nanoparticles as novel non-viral vectors for targeted gene delivery
    Roy, I
    Mitra, S
    Maitra, A
    Mozumdar, S
    INTERNATIONAL JOURNAL OF PHARMACEUTICS, 2003, 250 (01) : 25 - 33
  • [2] Nanostructured silicate substituted calcium phosphate (NanoSiCaPs) nanoparticles - Efficient calcium phosphate based non-viral gene delivery systems
    Shekhar, Sudhanshu
    Roy, Abhijit
    Hong, Daeho
    Kumta, Prashant N.
    MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2016, 69 : 486 - 495
  • [3] Nuclear localisation and pDNA condensation in non-viral gene delivery
    Collins, Elizabeth
    Birchall, James C.
    Williams, Julie L.
    Gumbleton, Mark
    JOURNAL OF GENE MEDICINE, 2007, 9 (04): : 265 - 274
  • [4] An efficient, non-viral dendritic vector for gene delivery in tissue engineering
    D P Walsh
    A Heise
    F J O’Brien
    S-A Cryan
    Gene Therapy, 2017, 24 : 681 - 691
  • [5] An efficient, non-viral dendritic vector for gene delivery in tissue engineering
    Walsh, D. P.
    Heise, A.
    O'Brien, F. J.
    Cryan, S-A
    GENE THERAPY, 2017, 24 (11) : 681 - 691
  • [6] A non-viral vector for in vivo gene delivery
    Thomas, L
    LANCET NEUROLOGY, 2005, 4 (10): : 602 - 602
  • [7] Novel nanostructured calcium phosphate based delivery systems for non-viral gene delivery
    Olton, Dana
    Lee, Dong Hyun
    Sfeir, Charles
    Kumta, Prashant N.
    PROCEEDING OF THE ASME SUMMER BIOENGINEERING CONFERENCE - 2007, 2007, : 1001 - 1002
  • [8] Non-viral gene delivery using nanoparticles
    Ditto, Andrew J.
    Shah, Parth N.
    Yun, Yang H.
    EXPERT OPINION ON DRUG DELIVERY, 2009, 6 (11) : 1149 - 1160
  • [9] Nanoparticles as Non-Viral Gene Delivery Vectors
    Katragadda, Chinmayee Sarita
    Choudhury, Prasanta Kumar
    Murthy, P. N.
    INDIAN JOURNAL OF PHARMACEUTICAL EDUCATION AND RESEARCH, 2010, 44 (02) : 109 - 120
  • [10] Polyethylenimine functionalized magnetic nanoparticles as a potential non-viral vector for gene delivery
    Yangbo Zhou
    Zhaomin Tang
    Chunli Shi
    Shuai Shi
    Zhiyong Qian
    Shaobing Zhou
    Journal of Materials Science: Materials in Medicine, 2012, 23 : 2697 - 2708
12345