Histone H2B Deacylation Selectivity: Exploring Chromatin's Dark Matter with an Engineered Sortase

被引：34

作者：

Wang, Zhipeng A. ^{[1
,2
]}

Whedon, Samuel D. ^{[1
,2
]}

Wu, Mingxuan ^{[1
,2
]}

Wang, Siyu ^{[3
]}

Brown, Edward A. ^{[3
]}

Anmangandla, Ananya ^{[4
]}

Regan, Liam ^{[3
]}

Lee, Kwangwoon ^{[1
,2
]}

Du, Jianfeng ^{[5
]}

Hong, Jun Young ^{[4
]}

Fairall, Louise ^{[3
]}

Kay, Taylor ^{[1
,2
]}

Lin, Hening ^{[4
]}

Zhao, Yingming ^{[5
]}

Schwabe, John W. R. ^{[3
]}

Cole, Philip A. ^{[1
,2
]}

机构：

[1] Brigham & Womens Hosp, Dept Med, Div Genet, Boston, MA 02115 USA

[2] Harvard Med Sch, Dept Biol Chem & Mol Pharmcol, Boston, MA 02115 USA

[3] Univ Leicester, Leicester Inst Struct & Chem Biol, Dept Mol & Cell Biol, Leicester LE1 7RH, Leics, England

[4] Cornell Univ, Howard Hughes Med Inst, Dept Chem & Chem Biol, Ithaca, NY 14853 USA

[5] Ben May Dept Canc Res, Chicago, IL 60637 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2022年 / 144卷 / 08期

基金：

英国惠康基金;

关键词：

METABOLIC-REGULATION; CHEMICAL-SYNTHESIS; GENE-EXPRESSION; DEACETYLASE; DEMETHYLATION; ACETYLATION; RECOGNITION; INHIBITORS; PROTEINS; BIOLOGY;

D O I：

10.1021/jacs.1c13555

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We describe a new method to produce histone H2B by semisynthesis with an engineered sortase transpeptidase. N-Terminal tail site-specifically modified acetylated, lactylated, and beta-hydroxybutyrylated histone H2Bs were incorporated into nucleosomes and investigated as substrates of histone deacetylase (HDAC) complexes and sirtuins. A wide range of rates and site-specificities were observed by these enzyme forms suggesting distinct biological roles in regulating chromatin structure and epigenetics.

引用

页码：3360 / 3364

页数：5

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