Cosensitization Strategy with Cascade Energy Level Arrangement for Ultrasensitive Photoelectrochemical Protein Detection

被引:23
|
作者
Zhang, Yan-Hui [1 ]
Zheng, Ying-Ning [1 ]
Li, Meng-Jie [1 ]
Hu, Tao [1 ]
Yuan, Ruo [1 ]
Wei, Sha-Ping [1 ]
机构
[1] Southwest Univ, Coll Chem & Chem Engn, Minist Educ, Key Lab Luminescent & Real Time Analyt Chem, Chongqing 400715, Peoples R China
关键词
POLYMER SOLAR-CELLS; SIGNAL AMPLIFICATION STRATEGY; HIGH-PERFORMANCE; QUANTUM DOTS; IMMUNOASSAY; BIOSENSOR; PLATFORM; DONOR; COPOLYMERS; POLYANILINE;
D O I
10.1021/acs.analchem.8b03740
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Here, a photoelectrochemical (PEC) biosensor was established by a cosensitization strategy with cascade energy level arrangement for ultrasensitive detection of prostate-specific antigen (PSA). The proposed cosensitization strategy was based on the well-matched energy level arrangement of four kinds of organic photoactive materials, in which poly{4,8-bis [5-(2-ethylhexyl)thiophen-2-yl] benzo 1,2-b:4,5-b']dithiophene-2,6-diyl-alt-3-fluoro-2-[(2-ethylhexyl)-carbonyl]thieno [3,4-b]thiophene-4,6-diyl} (PTB7-Th) was used as the photoactive material and perylenetetracarboxyl diimide (PDI), fullerene (nano-C-60), and polyaniline (PANI) were employed as the sensitizers. The resulting PTB7-Th/PDI/nano-C-60/PANI cascade cosensitization structure with narrow energy level gradient (<0.54 eV) could effectively improve electron transfer capability, obviously raise light energy utilization and significantly enhance photoelectric conversion efficiency, leading to dramatically enhanced photocurrent response. Using PSA as a target model, the proposed PEC biosensor exhibited high sensitivity and excellent stability with a wide detection range from 1 fg/mL to 0.1 ng/mL and a detection limit of 0.43 fg/mL. Moreover, the proposed PEC biosensor provides a cascade cosensitization strategy that could significantly improve PEC performances and open up a promising platform to establish high selectivity, stability, and ultrasensitive analytical techniques.
引用
收藏
页码:12278 / 12283
页数:6
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